Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Rational design of a solvatochromic fluorescent uracil analogue with a dual-band ratiometric response based on 3-hydroxychromone
Chemistry - A European Journal, Volume 20, No. 7, Year 2014
Notification
URL copied to clipboard!
Description
Fluorescent nucleoside analogues with strong and informative responses to their local environment are in urgent need for DNA research. In this work, the design, synthesis and investigation of a new solvatochromic ratiometric fluorophore compiled from 3-hydroxychromones (3HCs) and uracil fragments are reported. 3HC dyes are a class of multi-parametric, environment-sensitive fluorophores providing a ratiometric response due to the presence of two well-resolved bands in their emission spectra. The synthesized conjugate demonstrates not only the preservation but also the improvement of these properties. The absorption and fluorescence spectra are shifted to longer wavelengths together with an increase of brightness. Moreover, the two fluorescence bands are better resolved and provide ratiometric responses across a broader range of solvent polarities. To understand the photophysical properties of this new fluorophore, a series of model compounds were synthesized and comparatively investigated. The obtained data indicate that uracil and 3HC fragments of this derivative are coupled into an electronic conjugated system, which on excitation attains strong charge-transfer character. The developed fluorophore is a prospective label for nucleic acids. © 2014 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim.
Authors & Co-Authors
Barthès, Nicolas P.F.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Michel, Benoît Y.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Klymchenko, Andrey S.
France, Illkirch-graffenstaden
Laboratoire de Bioimagerie et Pathologies - Lbp
Benhida, Rachid
France, Paris
Cnrs Centre National de la Recherche Scientifique
Mély, Yves
France, Illkirch-graffenstaden
Laboratoire de Bioimagerie et Pathologies - Lbp
Burger, Alain C.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 45
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1002/chem.201303399
ISSN:
15213765
Research Areas
Environmental
Genetics And Genomics
Study Design
Cohort Study