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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Nicorandil decreases postischemic actin oxidation
Free Radical Biology and Medicine, Volume 31, No. 5, Year 2001
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Description
This study examined the hypothesis that preconditioning can decrease postischemic oxidative protein damage. Isolated rat hearts were subjected to 25 min of normothermic global ischemia followed by 45 min of reperfusion. These were compared with hearts pretreated with 20 μM nicorandil or preconditioned with two cycles of ischemia. Changes in the high energy phosphates, ATP and phosphocreatine, were followed using 31P-NMR spectroscopy. Protein carbonyls were assessed using an immunoblot technique. Postischemic hemodynamic function and high energy phosphates recovered to significantly (p < .05) higher levels in nicorandil-treated and ischemic preconditioned hearts as compared to controls. Postischemic protein carbonyl formation was highest in control reperfused hearts but reduced to intermediate between control and preischemic hearts by ischemic preconditioning and virtually prevented by nicorandil pretreatment, with a prominent band at 43 kDa significantly affected (p < .05). Based on immunoshift and immunoprecipitation studies, this band was identified as a mixture of actin isoforms. These studies support the conclusion that nicorandil diminishes protein oxidative damage in general, and specifically actin oxidation, which in the presence of improved supply of high energy phosphates, leads to enhanced postischemic contractile function. © 2001 Elsevier Science Inc.
Authors & Co-Authors
Schwalb, Herzl
Israel, Jerusalem
Hadassah University Medical Centre
Olivson, Abira
Israel, Jerusalem
Hadassah University Medical Centre
Li, Judy
United States, Mineola
Nyu Winthrop Hospital
Houminer, Esther
Israel, Jerusalem
Hadassah University Medical Centre
Wahezi, Sayed E.
United States, Mineola
Nyu Winthrop Hospital
Opie, LionelH H.
South Africa, Cape Town
Faculty of Health Sciences
Maulik, Dev
United States, Mineola
Nyu Winthrop Hospital
Borman, Joseph Bernard
Israel, Jerusalem
Hadassah University Medical Centre
Powell, Saul R.
United States, Mineola
Nyu Winthrop Hospital
Statistics
Citations: 20
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1016/S0891-5849(01)00620-7
ISSN:
08915849