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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Remote fingerstick blood collection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody testing
Archives of Pathology and Laboratory Medicine, Volume 145, No. 4, Year 2021
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Description
The rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has propelled the rapid development of serologic tests that can detect anti–SARS-CoV-2 antibodies. These have been used for studying the prevalence and spread of infection in different populations, and helping establish a recent diagnosis of coronavirus disease 2019 (COVID-19), and will likely be used to confirm humoral immunity after infection or vaccination. However, nearly all lab-based high-throughput SARS-CoV-2 serologic assays require a serum sample from venous blood draw, limiting their applications and scalability. Here, we present a method that enables large-scale SARS-CoV-2 serologic studies by combining self or office collection of fingerprick blood with a volumetric absorptive microsampling device (Mitra, Neoteryx LLC) with a high-throughput electrochemiluminescence-based SARS-CoV-2 total antibody assay (Roche Elecsys, Roche Diagnostics Inc) that is emergency use authorization approved for use on serum samples and widely used by clinical laboratories around the world. We found that the Roche Elecsys assay has a high dynamic range that allows for accurate detection of SARS-CoV-2 antibodies in serum samples diluted 1:20 as well as contrived dried blood extracts. Extracts of dried blood from Mitra devices acquired in a community seroprevalence study showed near identical sensitivity and specificity in detection of SARS-CoV-2 antibodies compared with neat sera using predefined thresholds for each specimen type. Overall, this study affirms the use of Mitra dried blood collection device with the Roche Elecsys SARS-CoV-2 total antibody assay for remote or at-home testing as well as large-scale community seroprevalence studies. © 2021 College of American Pathologists. All rights reserved.
Authors & Co-Authors
García-Beltrán, Wilfredo F.
United States, Boston
Massachusetts General Hospital
Miller, Tyler E.
United States, Boston
Massachusetts General Hospital
Kirkpatrick, Grace E.
United States, Boston
Massachusetts General Hospital
Astudillo, Michael Gerino
United States, Boston
Massachusetts General Hospital
Yang, Diane
United States, Boston
Massachusetts General Hospital
Murali, Mandakolathur Ramaswamy
United States, Boston
Massachusetts General Hospital
Dighe, Anand S.
United States, Boston
Massachusetts General Hospital
Lennerz, Jochen Klaus Maria
United States, Boston
Massachusetts General Hospital
Thierauf, Julia Cara
United States, Boston
Massachusetts General Hospital
Naranbhai, Vivek
United States, Boston
Massachusetts General Hospital
Statistics
Citations: 8
Authors: 10
Affiliations: 2
Identifiers
Doi:
10.5858/arpa.2020-0713-sa
ISSN:
00039985
Research Areas
Covid
Study Design
Cross Sectional Study