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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection and molecular identification of human adenoviruses and enteroviruses in wastewater from Morocco
Letters in Applied Microbiology, Volume 54, No. 4, Year 2012
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Description
Aims: Reclaimed wastewater is a considerable water resource in Morocco. Its agricultural reuse requires an assessment of viral contamination. The aim of this study was to detect both infectious and noninfectious human adenoviruses (HAdV) and enteroviruses (EV) in raw wastewater and treat effluent from wastewater treatment plants (WWTPs) and domestic sewage in Morocco. Methods and Results: A total of 22 samples were analysed. A polyethylene glycol (PEG) precipitation method was used, followed by integrated cell culture-PCR (ICC-PCR) using two cell lines: human rhabdomyosarcoma tumour tissue and laryngeal carcinoma cells (RD and Hep2 cells). Furthermore, viral genome amplification was confirmed by sequencing. HAdV were detected in 10 (45·5%) of the 22 samples involving two species: HAdV-B and HAdV-D. EV was detected in 5 (23%) samples belonging to Coxsackievirus B5 and poliovirus vaccine strain (Sabin 2). Conclusions: Human adenoviruses and EV were detected in the analysed samples from two WWTPs and HAdV in domestic sewage. Significance and Impact of the Study: This work is the first study in Morocco using cell culture, PCR and sequencing of enteric viruses from wastewater. The presence of infectious HAdV and EV in treated effluent emphasizes the need of wastewater treatment surveillance. © 2012 The Authors. Letters in Applied Microbiology © 2012.
Authors & Co-Authors
Amdiouni, Hasna
Morocco, Casablanca
Institut Pasteur du Maroc
Morocco, Casablanca
Laboratoire de Physiologie et Génétique Moléculaire
Faouzi, Abdellah
Morocco, Casablanca
Institut Pasteur du Maroc
Fariat, N.
Morocco, Casablanca
Institut Pasteur du Maroc
Hassar, Mohammed
Morocco, Casablanca
Institut Pasteur du Maroc
Soukri, Abdelaziz
Morocco, Casablanca
Laboratoire de Physiologie et Génétique Moléculaire
Nourlil, Jalal
Morocco, Casablanca
Institut Pasteur du Maroc
Statistics
Citations: 26
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1111/j.1472-765X.2012.03220.x
ISSN:
02668254
e-ISSN:
1472765X
Research Areas
Cancer
Environmental
Study Locations
Morocco