Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Profiling the specificity of neutralizing antibodies in a large panel of plasmas from patients chronically infected with human immunodeficiency virus type 1 subtypes B and C
Journal of Virology, Volume 82, No. 23, Year 2008
Notification
URL copied to clipboard!
Description
Identifying the viral epitopes targeted by broad neutralizing antibodies (NAbs) that sometimes develop in human immunodeficiency virus type 1 (HIV-1)-infected subjects should assist in the design of vaccines to elicit similar responses. Here, we investigated the activities of a panel of 24 broadly neutralizing plasmas from subtype B- and C-infected donors using a series of complementary mapping methods, focusing mostly on JR-FL as a prototype subtype B primary isolate. Adsorption with gp120 immobilized on beads revealed that an often large but variable fraction of plasma neutralization was directed to gp120 and that in some cases, neutralization was largely mediated by CD4 binding site (CD4bs) Abs. The results of a native polyacrylamide gel electrophoresis assay using JR-FL trimers further suggested that half of the subtype B and a smaller fraction of subtype C plasmas contained a significant proportion of NAbs directed to the CD4bs. Anti-gp41 neutralizing activity was detected in several plasmas of both subtypes, but in all but one case, constituted only a minor fraction of the overall neutralization activity. Assessment of the activities of the subtype B plasmas against chimeric HIV-2 viruses bearing various fragments of the membrane proximal external region (MPER) of HIV-1 gp41 revealed mixed patterns, implying that MPER neutralization was not dominated by any single specificity akin to known MPER-specific monoclonal Abs. V3 and 2G12-like NAbs appeared to make little or no contribution to JR-FL neutralization titers. Overall, we observed significant titers of anti-CD4bs NAbs in several plasmas, but approximately two-thirds of the neutralizing activity remained undefined, suggesting the existence of NAbs with specificities unlike any characterized to date. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S1.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S2.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S3.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S4.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S5.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__Figure__S6_use.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__figure_S7use.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2583680/bin/supp_82_23_11651__Supplemental_Figure_Legends.doc
Authors & Co-Authors
Binley, James M.
United States, San Diego
Torrey Pines Institute for Molecular Studies
Lybarger, Elizabeth A.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Crooks, Emma T.
United States, San Diego
Torrey Pines Institute for Molecular Studies
Seaman, Michael S.
United States, Boston
Beth Israel Deaconess Medical Center
Gray, Elin Solomonovna
South Africa, Johannesburg
National Institute for Communicable Diseases
Davis, Katie L.
United States, Birmingham
The University of Alabama at Birmingham
Decker, Julie M.
United States, Birmingham
The University of Alabama at Birmingham
Wycuff, Diane R.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Harris, Linda J.
United States, Seattle
Fred Hutchinson Cancer Research Center
Hawkins, Natalie R.
United States, Seattle
Fred Hutchinson Cancer Research Center
Wood, Blake
United States, Seattle
Fred Hutchinson Cancer Research Center
Nathe, Cory
United States, Seattle
Fred Hutchinson Cancer Research Center
Richman, Douglas D.
United States, La Jolla
University of California, San Diego
Tomaras, Georgia D.
United States, Durham
Duke University Medical Center
Bibollet-Ruche, Frédéric
United States, Birmingham
The University of Alabama at Birmingham
Robinson, James E.
United States, New Orleans
Tulane University School of Medicine
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Shaw, George M.
United States, Birmingham
The University of Alabama at Birmingham
Montefiori, David Charles
United States, Durham
Duke University Medical Center
Mascola, John R.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 365
Authors: 20
Affiliations: 9
Identifiers
Doi:
10.1128/JVI.01762-08
ISSN:
0022538X
Research Areas
Infectious Diseases