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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
C-Terminal tensin-like gene functions as an oncogene and promotes cell motility in pancreatic cancer
Pancreas, Volume 42, No. 1, Year 2013
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Description
OBJECTIVES: C-terminal tensin-like gene (CTEN, also known as TNS4) localizes to focal adhesions and is reported to function as an oncogene in colonic, breast, lung, and gastric cancers. Its role in pancreatic cancer is unknown and was thus investigated in this study. METHODS: C-terminal tensinlike gene expression was evaluated by immunohistochemistry in a series of pancreatic cancers. Functional activity of the CTEN was tested by manipulating cellular CTEN levels using a dual approach of gene knockdown/forced expression. RESULTS: The CTEN is overexpressed in 31 (70.45%) of 44 pancreatic cancers. Functionally, changes in CTEN level did not alter cellular proliferation, but CTEN levels were positively associated with enhanced colony-forming efficiency in both Panc-1 and PSN-1 cell lines. Forced CTEN expression in Panc-1 cells stimulated cell motility, whereas knockdown of CTEN in PSN-1 inhibited cell motility in both transwell migration and wound-healing assays. Evaluation of downstream targets demonstrated that alterations in CTEN levels induced changes in focal adhesion kinase and E-cadherin, whereas integrin-linked kinase (ILK) remained unchanged. CONCLUSIONS: These are the first data showing an oncogenic role for CTEN in pancreatic cancer through promotion of colony formation and cell motility. The latter may be mediated by signaling through focal adhesion kinase and inhibiting E-cadherin. Copyright © 2012 by Lippincott Williams & Wilkins.
Authors & Co-Authors
Al-Ghamdi, Saleh
United Kingdom, Nottingham
Queen's Medical Centre
Saudi Arabia, Riyadh
King Saud Bin Abdulaziz University for Health Sciences
Cachat, Julien
United Kingdom, Nottingham
Queen's Medical Centre
Albasri, Abdulkader Mohammed
United Kingdom, Nottingham
Queen's Medical Centre
Ahmed, Mohamed A.H.
United Kingdom, Nottingham
Queen's Medical Centre
Jackson, Darryl
United Kingdom, Nottingham
Queen's Medical Centre
Zaitoun, Abed M.
United Kingdom, Nottingham
Queen's Medical Centre
Guppy, Naomi Jayne
United Kingdom, London
Barts and the London School of Medicine and Dentistry
Otto, William R.
United Kingdom, London
Cancer Research uk
Alison, Malcolm Ronald
United Kingdom, London
Cancer Research uk
Kindle, Karin B.
United Kingdom, Nottingham
Queen's Medical Centre
Ilyas, Mohammad
United Kingdom, Nottingham
Queen's Medical Centre
Statistics
Citations: 34
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1097/MPA.0b013e3182557ceb
ISSN:
08853177
e-ISSN:
15364828
Research Areas
Cancer
Genetics And Genomics