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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Expression of the Domain Cassette 8 Plasmodium falciparum Erythrocyte Membrane Protein 1 Is Associated with Cerebral Malaria in Benin
PLoS ONE, Volume 8, No. 7, Article e68368, Year 2013
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Description
Background:Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1) is a highly polymorphic adherence receptor expressed on the surface of infected erythrocytes. Based on sequence homology PfEMP-1 variants have been grouped into three major groups A-C, the highly conserved VAR2CSA variants, and semi-conserved types defined by tandem runs of specific domains ("domain cassettes" (DC)). The PfEMP-1 type expressed determines the adherence phenotype, and is associated with clinical outcome of infection.Methods:Parasite isolates from Beninese children or women presenting with, respectively, CM or PAM were collected along with samples from patients with uncomplicated malaria (UM). We assessed the transcript level of var genes by RT-qPCR and the expression of PfEMP-1 proteins by LC-MS/MS.Results:Var genes encoding DC8 and Group A PfEMP-1 were transcribed more often and at higher levels in cerebral malaria vs. uncomplicated malaria patients. LC-MS/MS identified peptides from group A, DC8 PfEMP-1 more frequently in cerebral malaria than in uncomplicated malaria and pregnancy-associated malaria samples.Conclusion:This is the first study to show association between PfEMP-1 subtype and disease outcome by direct analysis of parasites proteome. The results corroborate that group A and specifically the PfEMP-1 types DC8 are universally associated with cerebral malaria. This is a crucial observation for promoting studies on malaria pathogenesis. © 2013 Bertin et al.
Authors & Co-Authors
Bertin, Gwladys I.
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Lavstsen, Thomas
Denmark, Copenhagen
Københavns Universitet
Guillonneau, François
France, Paris
Université Paris Cité
Doritchamou, Justin
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Benin
Centre D'etude et de Recherche Sur le Paludisme Associé à la Grossesse et L'enfance Cerpage
Wang, Christian William
Denmark, Copenhagen
Københavns Universitet
Jespersen, Jakob S.
Denmark, Copenhagen
Københavns Universitet
Ezinmègnon, Sèm
Benin
Centre D'etude et de Recherche Sur le Paludisme Associé à la Grossesse et L'enfance Cerpage
Fievét, Nadine
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Benin
Centre D'etude et de Recherche Sur le Paludisme Associé à la Grossesse et L'enfance Cerpage
Alao, Maroufou Jules
Benin, Cotonou
Mother and Child Hospital Homel
Lalya, Francis
Benin, Cotonou
Centre National Hospitalo-universitaire Cnhu
Massougbodji, Achille
Benin
Centre D'etude et de Recherche Sur le Paludisme Associé à la Grossesse et L'enfance Cerpage
Tuikue-Ndam, Nicaise
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Benin
Centre D'etude et de Recherche Sur le Paludisme Associé à la Grossesse et L'enfance Cerpage
G. Theander, Thor Grundtvig
Denmark, Copenhagen
Københavns Universitet
Deloron, Philippe
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Statistics
Citations: 65
Authors: 14
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0068368
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Sexual And Reproductive Health
Study Locations
Benin
Participants Gender
Female