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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
MLVA polymorphism of Salmonella enterica subspecies isolated from humans, animals, and food in Cambodia
BMC Research Notes, Volume 4, Article 306, Year 2011
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Description
Background: Salmonella (S.) enterica is the main cause of salmonellosis in humans and animals. The epidemiology of this infection involves large geographical distances, and strains related to an episode of salmonellosis therefore need to be reliably discriminated. Due to the limitations of serotyping, molecular genotyping methods have been developed, including multiple loci variable number of tandem repeats (VNTR) analysis (MLVA). In our study, 11 variable number tandem-repeats markers were selected from the S. enterica Typhimurium LT2 genome to evaluate the genetic diversity of 206 S. enterica strains collected in Cambodia between 2001 and 2007. Findings. Thirty one serovars were identified from three sources: humans, animals and food. The markers were able to discriminate all strains from 2 to 17 alleles. Using the genotype phylogeny repartition, MLVA distinguished 107 genotypes clustered into two main groups: S. enterica Typhi and other serovars. Four serovars (Derby, Schwarzengrund, Stanley, and Weltevreden) were dispersed in 2 to 5 phylogenic branches. Allelic variations within S. enterica serovars was represented using the minimum spanning tree. For several genotypes, we identified clonal complexes within the serovars. This finding supports the notion of endemo-epidemic diffusion within animals, food, or humans. Furthermore, a clonal transmission from one source to another was reported. Four markers (STTR3, STTR5, STTR8, and Sal20) presented a high diversity index (DI > 0.80). Conclusions: In summary, MLVA can be used in the typing and genetic profiling of a large diversity of S. enterica serovars, as well as determining the epidemiological relationships of the strains with the geography of the area. © 2011 Kruy et al; licensee BioMed Central Ltd.
Authors & Co-Authors
van Cuyck, Hélène
France, Villenave-d'ornon
Hôpital D'instruction Des Armées Robert Picqué
Leroy, Philippe
France, Villenave-d'ornon
Hôpital D'instruction Des Armées Robert Picqué
Vuthy, Yith
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Guillard, Bertrand
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Sarthou, Jean Louis
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Koeck, Jean Louis
France, Villenave-d'ornon
Hôpital D'instruction Des Armées Robert Picqué
Kruy, Sun Lay
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Statistics
Citations: 15
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1186/1756-0500-4-306
ISSN:
17560500
Research Areas
Food Security
Genetics And Genomics
Infectious Diseases