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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
First detection and sequence analysis of the bla-CTX-M-15 gene in Lebanese isolates of extended-spectrum-β-lactamase-producing Shigella sonnei
Annals of Tropical Medicine and Parasitology, Volume 101, No. 6, Year 2007
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Description
The emergence in Shigella species of extended-spectrum beta-lactamases (ESBL) that impart resistance to third-generation cephalosporins is a growing concern world-wide. So far, however, ESBL-producing Shigella have only been reported seven times, albeit from seven different countries. In Lebanon, three ESBL-producing clinical isolates of S. sonnei were recovered from 30 cases of shigellosis diagnosed between July 2004 and October 2005. All three were found to be resistant to amoxycillin, cefotaxime, ceftazidime, aztreonam, trimethoprim/sulphamethoxazole, gentamicin, and kanamycin. Each harboured the bla-CTX-M gene, and the results of sequence analysis indicated this to be of the bla-CTX-M-15 type and encoded on a 70-kb plasmid, flanked by an insertion element (ISEcpl1. The bla-TEM-1 gene was also detected on the chromosomes of two of the ESBL-producing isolates. Class-2 integrons containing dhfr1, aadA1 and sat1 genes were detected on the chromosomes of all three isolates but not on the plasmids. Fluoroquinolone-modifying factors [QnrA, QnrB, QnrS or AAC(6')-Ib-cr] were not detected. The results of RAPD analysis, combined with data on antimicrobial susceptibility, indicated that each isolate was unique. In conclusion, the emergence of ESBL-producing isolates of S. sonnei has been demonstrated for the first time in Lebanon. The resistance of these isolates to third-generation cephalosporins was mediated by the CTX-M-15 enzyme, which was plasmid-encoded. © 2007 The Liverpool School of Tropical Medicine.
Authors & Co-Authors
Matar, Ghassan
Lebanon, Beirut
American University of Beirut
Jaafar, Rola F.
Lebanon, Beirut
American University of Beirut
Sabra, Ahmad H.
Lebanon, Beirut
American University of Beirut
Hart, Charles Anthony
United Kingdom, Liverpool
University of Liverpool
Corkill, John Edward
United Kingdom, Liverpool
University of Liverpool
Dbaibo, Ghassan S.
Lebanon, Beirut
American University of Beirut
Araj, George F.
Lebanon, Beirut
American University of Beirut
Statistics
Citations: 35
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1179/136485907X193860
ISSN:
00034983
Research Areas
Genetics And Genomics
Health System And Policy