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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Does neutralization of gastric aspirates from children with suspected intrathoracic tuberculosis affect mycobacterial yields on MGIT culture?
Journal of Clinical Microbiology, Volume 51, No. 6, Year 2013
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Description
The microbiological confirmation of pulmonary tuberculosis in children relies on cultures of gastric aspirate (GA) specimens. Conventionally, GAs are neutralized to improve culture yields of mycobacteria. However, there are limited data to support this practice. To study the utility of neutralization of GAs with sodium bicarbonate in children with intrathoracic tuberculosis, a total of 116 children of either sex, aged 6 months to 14 years (median age, 120 months; interquartile range [IQR], 7 to 192 months), underwent gastric aspiration on 2 consecutive days. Gastric aspirates were divided into two aliquots, and only one aliquot was neutralized with 1% sodium bicarbonate. Both aliquots were processed for smear and culture examinations. Out of the 232 gastric aspirates, 12 (5.17%) were acid-fast bacilli (AFB) smear positive. There were no differences in smear positivity rates from samples with or without neutralization. The yield of Mycobacterium tuberculosis on a Bactec MGIT 960 culture system was significantly lower in the neutralized samples (16.3% [38/232]) than in the nonneutralized samples (21.5% [50/232]) ( P = 0.023). There was no significant difference between the neutralized and the nonneutralized samples in time to detection using the MGIT 960 system (average, 24.6 days; IQR, 12 to 37 days) (P = 0.9). The contamination rates were significantly higher in the neutralized samples than in the nonneutralized samples (17.2% [40/232] versus 3.9% [9/232]) (P = 0.001). The agreement for positive mycobacterial culture between the two approaches was 66.5% (P = 0.001). Hence, we recommend that gastric aspirate samples not be neutralized with sodium bicarbonate prior to culture for M. tuberculosis. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Kabra, Susheel Kumar
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Lodha, Rakesh K.
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Singh, Varinder P.
India, New Delhi
Kalawati Saran Children's Hospital
Mukherjee, Aparna Lisa
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Grewal, Harleen M.S.
Norway, Bergen
Gade Institute
Norway, Bergen
Haukeland Universitetssjukehus
Singh, Sarman M.
Unknown Affiliation
Bhatnagar, Shinjini
Unknown Affiliation
Aneja, Satinder
Unknown Affiliation
Marais, Ben J.
Unknown Affiliation
Hesseling, Anneke Catharina
Unknown Affiliation
Friis, Henrik M.U.
Unknown Affiliation
Doherty, Timothy Mark
Unknown Affiliation
Statistics
Citations: 28
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1128/JCM.00202-13
ISSN:
1098660X
Research Areas
Maternal And Child Health