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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
materials science
Capacity of mesoporous bioactive glass nanoparticles to deliver therapeutic molecules
Nanoscale, Volume 4, No. 23, Year 2012
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Description
Inorganic bioactive nanomaterials are attractive for hard tissue regeneration, including nanocomponents for bone replacement composites and nanovehicles for delivering therapeutics. Bioactive glass nanoparticles (BGn) have recently gained potential usefulness as bone and tooth regeneratives. Here we demonstrate the capacity of the BGn with mesopores to load and deliver therapeutic molecules (drugs and particularly genes). Spherical BGn with sizes of 80-90 nm were produced to obtain 3-5 nm sized mesopores through a sono-reacted sol-gel process. A simulated body fluid test of the mesoporous BGn confirmed their excellent apatite forming ability and the cellular toxicity study demonstrated their good cell viability up to 100 μg ml-1. Small molecules like chemical drug (Na-ampicillin) and gene (small interfering RNA; siRNA) were introduced as model drugs considering the mesopore size of the nanoparticles. Moreover, amine-functionalization allowed switchable surface charge property of the BGn (from -20-30 mV to +20-30 mV). Loading of ampicillin or siRNA saturated within a few hours (∼2 h) and reflected the mesopore structure. While the ampicillin released relatively rapidly (∼12 h), the siRNA continued to release up to 3 days with almost zero-order kinetics. The siRNA-nanoparticles were easily taken up by the cells, with a transfection efficiency as high as ∼80%. The silencing effect of siRNA delivered from the BGn, as examined by using bcl-2 model gene, showed dramatic down-regulation (∼15% of control), suggesting the potential use of BGn as a new class of nanovehicles for genes. This, in conjunction with other attractive properties, including size- and mesopore-related high surface area and pore volume, tunable surface chemistry, apatite-forming ability, good cell viability and the possible ion-related stimulatory effects, will potentiate the usefulness of the BGn in hard tissue regeneration. © 2012 The Royal Society of Chemistry.
Authors & Co-Authors
El-Fiqi, Ahmed M.
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Egypt, Giza
National Research Centre
Kim, T. H.
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Kim, Meeju
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Eltohamy, Mohamed R.
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Egypt, Giza
National Research Centre
Won, Jong Eun
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Lee, Eun Jung
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Kim, Hae-won
South Korea, Seoul
Dankook University
South Korea, Cheonan
Dankook University, Institute of Tissue Regeneration Engineering
Egypt, Giza
National Research Centre
South Korea, Cheonan
Dankook University, College of Dentistry
Statistics
Citations: 126
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1039/c2nr31775c
ISSN:
20403364
e-ISSN:
20403372
Research Areas
Genetics And Genomics