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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The hemizona assay (HZA): A predictor of human sperm fertilizing potential in in vitro fertilization (IVF) treatment
Journal of In Vitro Fertilization and Embryo Transfer, Volume 6, No. 1, Year 1989
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Description
The hemizona assay (HZA) was developed to assess human sperm fertilizing potential. This blinded study investigated the relationship between sperm binding to the hemizona and in vitro fertilization (IVF) success (36 patients). Nonliving human oocytes were recovered from excised ovaries and stored. Each zona pellucida was cut into equal hemispheres by micromanipulation. For the HZA, one droplet exposed a hemizona to abnormal spermatozoa, while the control droplet contained the matching hemizona and spermatozoa from normal semen. After 4 hr, the number of tightly bound spermatozoa was counted. Binding to the hemizona was significantly higher for those having IVF success (mean of 36.1±7, versus 10.4±4 from the failure group;P<0.05). Fewer sperm from the failure group had a strictly normal morphology (3,2 versus 12.7%;P<0.05, Kruger method). Tight zona binding was significantly correlated with the percentage motile sperm, percentage normal morphology, and seminal sperm concentration. These results enhanced our confidence that the HZA is diagnostic for identification of patients at high risk of failing to achieve fertilization in vitro. © 1989 Plenum Publishing Corporation.
Authors & Co-Authors
Franken, Daniel Rossouw
South Africa, Bloemfontein
Free State University
Oehninger, Sergio Carlos
United States, Norfolk
Eastern Virginia Medical School
Johnson Burkman, Lani
United States, Norfolk
Eastern Virginia Medical School
Coddington, Charles Campbell
United States, Portsmouth
Naval Medical Center - Portsmouth
Kruger, Theunis Frans
South Africa, Tygerberg
Tygerberg Hospital
Rosenwaks, Zev
United States, Norfolk
Eastern Virginia Medical School
Acosta, Aníbal A.
United States, Norfolk
Eastern Virginia Medical School
Hodgen, Gary D.
United States, Norfolk
Eastern Virginia Medical School
Statistics
Citations: 47
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1007/BF01134581
ISSN:
07407769
Study Design
Case-Control Study