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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Proteomic analysis of leaf proteins during dehydration of the resurrection plant Xerophyta viscosa
Plant, Cell and Environment, Volume 30, No. 4, Year 2007
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Description
The desiccation-tolerant phenotype of angiosperm resurrection plants is thought to rely on the induction of protective mechanisms that maintain cellular integrity during water loss. Two-dimensional (2D) sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the Xerophyta viscosa Baker proteome was carried out during dehydration to identify proteins that may play a role in such mechanisms. Quantitative analysis revealed a greater number of changes in protein expression levels at 35% than at 65% relative water content (RWC) compared to fully hydrated plants, and 17 dehydration-responsive proteins were identified by tandem mass spectrometry (MS). Proteins showing increased abundance during drying included an RNA-binding protein, chloroplast FtsH protease, glycolytic enzymes and antioxidants. A number of photosynthetic proteins declined sharply in abundance in X. viscosa at RWC below 65%, including four components of photosystem II (PSII), and Western blot analysis confirmed that two of these (psbP and Lhcb2) were not detectable at 30% RWC. These data confirm that poikilochlorophylly in X. viscosa involves the breakdown of photosynthetic proteins during dismantling of the thylakoid membranes. In contrast, levels of these photosynthetic proteins were largely maintained during dehydration in the homoiochlorophyllous species Craterostigma plantagineum Hochst, which does not dismantle thylakoid membranes on drying. © 2007 The Authors.
Authors & Co-Authors
Ingle, Robert A.
South Africa, Cape Town
University of Cape Town
Schmidt, Ulrike G.
Switzerland, Zurich
Universität Zürich
Farrant, Jill M.
South Africa, Cape Town
University of Cape Town
Thomson, Jennifer Ann
South Africa, Cape Town
University of Cape Town
Mundree, Sagadevan Govindasamy
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 125
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1111/j.1365-3040.2006.01631.x
ISSN:
01407791
Research Areas
Environmental
Study Approach
Quantitative