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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes
American Journal of Tropical Medicine and Hygiene, Volume 74, No. 5, Year 2006
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Description
We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W. bancrofti "LDR" repeat DNA sequence was more sensitive than an assay for Wolbachia 16S rDNA. The LDR-based assay was sensitive for detecting microfilarial DNA on dried membrane filters or on filter paper. We also compared real-time PCR with conventional PCR (C-PCR) for detecting W. bancrofti DNA in mosquito samples collected in endemic areas in Egypt and Papua New Guinea. Although the two methods had comparable sensitivity for detecting filarial DNA in reference samples, real-time PCR was more sensitive than C-PCR in practice with field samples. Other advantages of real-time PCR include its high-throughput capacity and decreased risk of cross-contamination between test samples. We believe that real-time PCR has great potential as a tool for monitoring progress in large-scale filariasis elimination programs. Copyright © 2006 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Rao, Ramakrishna
United States, St. Louis
Washington University School of Medicine in St. Louis
Atkinson, Laura J.
United States, St. Louis
Washington University School of Medicine in St. Louis
Ramzy, Reda M.R.
Egypt, Cairo
Ain Shams University
Helmy, Hanan H.
Egypt, Cairo
Ain Shams University
Farid, H. A.
Egypt, Cairo
Ain Shams University
Bockarie, Moses John
Papua new Guinea, Goroka
Papua new Guinea Institute of Medical Research
United States, Cleveland
Case School of Medicine
Susapu, Melinda
Papua new Guinea, Goroka
Papua new Guinea Institute of Medical Research
Laney, Sandra J.
United States, Northampton
Smith College
Williams, Steven A.
United States, Northampton
Smith College
Weil, Gary J.
United States, St. Louis
Washington University School of Medicine in St. Louis
Statistics
Citations: 140
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.4269/ajtmh.2006.74.826
ISSN:
00029637
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Locations
Egypt
Guinea