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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Strain-dependent viral dynamics and virus-cell interactions in a novel in vitro system supporting the life cycle of blood-borne hepatitis C Virus
Hepatology, Volume 50, No. 3, Year 2009
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Description
We developed an in vitro system that can be used for the study of the life cycle of a wide variety of blood-borne hepatitis C viruses (HCV) from various patients using a three-dimensional hollow fiber culture system and an immortalized primary human hepatocyte (HuS-E/2) cell line. Unlike the conventional two-dimensional culture, this system not only enhanced the infectivity of blood-borne HCV but also supported its long-term proliferation and the production of infectious virus particles. Both sucrose gradient fractionation and electron microscopy examination showed that the produced virus-like particles are within a similar fraction and size range to those previously reported. Infection with different HCV strains showed strain-dependent different patterns of HCV proliferation and particle production. Fluctuation of virus proliferation and particle production was found during prolonged culture and was found to be associated with change in the major replicating virus strain. Induction of cellular apoptosis was only found when strains of HCV-2a genotype were used for infection. Interferon-alpha stimulation also varied among different strains of HCV-1b genotypes tested in this study. Conclusion: These results suggest that this in vitro infection system can reproduce strain-dependent events reflecting viral dynamics and viruscell interactions at the early phase of blood-borne HCV infection, and that this system can allow the development of new anti-HCV strategies specific to various HCV strains. Copyright © 2009 by the American Association for the Study of Liver Diseases.
Authors & Co-Authors
Aly, Hussein H.
Japan, Kyoto
Kyoto University
Egypt, Cairo
National Hepatology and Tropical Medicine Research Institute
Qi, Yue
Japan, Kyoto
Kyoto University
Atsuzawa, Kimie
Japan, Toyoake
Fujita Health University School of Medicine
Usuda, Nobuteru
Japan, Toyoake
Fujita Health University School of Medicine
Takada, Yasutugu
Japan, Kyoto
Kyoto University
Mizokami, Masashi
Japan, Ichikawa
Japan Kounodai Hospital
Shimotohno, Kunitada
Japan, Tokyo
Keio University
Hijikata, Makoto
Japan, Kyoto
Kyoto University
Statistics
Citations: 21
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1002/hep.23034
ISSN:
02709139
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases