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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Transcriptome changes in culex quinquefasciatus (Diptera: Culicidae) salivary glands during west nile virus infection
Journal of Medical Entomology, Volume 47, No. 3, Year 2010
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Description
Persistent West Nile virus (WNV) infection in the mosquito Culex quinquefasciatus Say (Diptera: Culicidae) is associated with pathological changes in the salivary glands, including apoptotic cell death and a corresponding reduction in virus transmission over time. The vector host response to WNV infection and the molecular basis of WNV pathogenesis in Cx. quinquefasciatus was investigated using oligonucleotide microarrays designed to detect differences in the salivary gland transcriptome between WNV-infected mosquitoes and uninfected controls. Transcripts with increased abundance in infected salivary glands included those related to immunity, transcription, protein transport and degradation, amino acid and nucleotide metabolism, signal transduction, and cellular detoxification. Microarray-based analysis detected a decrease in transcript levels of a Culex inhibitor of apoptosis gene (IAP-1) and a decrease in abundance of 11 transcripts encoding salivary gland proteins, Transcript levels for an endonuclease, a proline-rich mucin, and several D7 protein family members also decreased. Transcripts with the greatest change in abundance during infection had either no similarity to sequences found in GenBank, VectorBase, and FlyBase, or were similar to sequences with uncharacterized protein products. These transcripts represent exciting targets for future analysis. Results from this study suggest that WNV infection influences transcriptional changes in an invertebrate host target tissue that may confer an advantage to the replicating virus, induce a host defense response, and alter the composition of vector saliva. The ramifications of these changes are discussed in terms of mosquito vector competence and WNV pathogenesis. © 2010 Entomological Society of America.
Authors & Co-Authors
Girard, Yvette A.
United States, Galveston
University of Texas Medical Branch, School of Medicine
United States, Davis
School of Veterinary Medicine
Mayhew, George
United States, Madison
University of Wisconsin-madison
Fuchs, Jeremy F.
United States, Madison
University of Wisconsin-madison
Li, Huarong
Egypt, Cairo
Ain Shams University
Schneider, Bradley S.
United States, Galveston
University of Texas Medical Branch, School of Medicine
France, Paris
Institut Pasteur, Paris
McGee, Charles E.
United States, Galveston
University of Texas Medical Branch, School of Medicine
Rocheleau, Thomas A.
United States, Madison
University of Wisconsin-madison
Helmy, Hanan H.
Egypt, Cairo
Ain Shams University
Christensen, Bruce M.
United States, Madison
University of Wisconsin-madison
Higgs, Stephen T.
United States, Galveston
University of Texas Medical Branch, School of Medicine
Bartholomay, Lyric C.
Unknown Affiliation
Statistics
Citations: 42
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1603/ME09249
ISSN:
00222585
Research Areas
Cancer
Genetics And Genomics