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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney
Nephrology Dialysis Transplantation, Volume 25, No. 6, Year 2010
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Description
Background. Tyrosine phosphorylation of proteins has been a focus of extensive studies since it plays crucial roles in regulation of diverse biological reactions. To understand the involvement of tyrosine phosphorylation in kidney functions, a comprehensive proteomic study for tyrosine-phosphorylated proteins was performed in the normal rat kidney.Methods. Two-dimensional gel electrophoresis and immunoprecipitation using anti-phosphotyrosine antibodies were employed to detect tyrosine-phosphorylated proteins. The proteins were analysed by mass spectrometry and validated by immunological analyses using specific antibodies.Results. Most of tyrosine-phosphorylated proteins were confined to the glomerulus and predominantly localized along the glomerular capillary wall, especially in the foot processes of podocytes. Our systematic proteomic analysis identified nephrin, SHPS-1 (tyrosine-protein phosphatase non-receptor-type substrate 1), FAK1 and paxillin as major tyrosine- phosphorylated proteins and Neph1, talin and vinculin as minor tyrosine-phosphorylated proteins. In the present study, SHPS-1 was identified as a novel tyrosine-phosphorylated protein in the glomerulus and was also predominantly localized at the foot processes. Mass spectrometric analysis identified in vivo phosphorylation sites of SHPS-1 on Y460, Y477 and Y501.Conclusion. This study identified tyrosine-phosphorylated proteins in normal rat kidney, which were prominently rich in the glomerulus and localized at the podocyte foot processes. These proteins were categorized as cell-to-cell or cell-to-matrix adhesion complex-related molecules, suggesting their pivotal roles in the glomerular ultrafiltration. © 2010 The Author.
Authors & Co-Authors
Zhang, Yingying
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Yoshida, Yutaka
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Nameta, Masaaki
Japan, Niigata
Niigata University
Xu, Bo
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Ikeda, Takako
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Fujinaka, Hidehiko
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Japan, Niigata
National Hospital Organization Niigata National Hospital
Magdeldin, Sameh
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Tsukaguchi, Hiroyasu
Japan, Tokushima
Graduate School of Medical Sciences
Japan, Tokyo
The University of Tokyo
Yaoita, Eishin
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Yamamoto, Tadashi S.
Japan, Tokyo
Graduate School of Medical and Dental Sciences
Statistics
Citations: 22
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1093/ndt/gfp697
ISSN:
09310509