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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Prediction of pH-dependent properties of DNA triple helices
Archives of Biochemistry and Biophysics, Volume 317, No. 1, Year 1995
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Description
The thermodynamic properties of two triple helices were investigated by uv thermal denaturation, differential scanning calorimetry, and pH titrations. Starting from the grand partition function and using matrix methods we present a formalism that describes pH effects on the thermal stability of triple helices. The formalism can be used over a wide pH range and is not restricted to the limiting case where the pH is larger or smaller than the pK(α) of cytosine. Furthermore, it covers nearest neighbor electrostatic effects of closely spaced cytosines in the Hoogsteen strand which can shift the pK(α) of cytosine to lower pH values. A procedure is employed to predict enthalpy and entropy changes for triplex formation. These values are in accordance with the results obtained by differential scanning calorimetry. © 1995 Academic Press, Inc.
Authors & Co-Authors
Hüsler, Paul L.
South Africa, Cape Town
University of Cape Town
Klump, Horst H.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 26
Authors: 2
Affiliations: 1
Identifiers
Doi:
10.1006/abbi.1995.1134
ISSN:
00039861
Research Areas
Genetics And Genomics