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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
The use of the modified hypo-osmotic swelling test for the selection of immotile testicular spermatozoa in patients treated with ICSI: A randomized controlled study
Human Reproduction, Volume 20, No. 12, Year 2005
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Description
Background: Various methods for the selection of viable sperm from among immotile testicular spermatozoa for use in ICSI have been described in non-randomized studies. We have conducted a randomized controlled study to compare the use of the modified hypo-osmotic swelling (HOS) test (50% culture medium + 50% Milli-Q grade water) with that of sperm selection on the basis of their morphology alone. Methods: A total of 79 couples with immotile testicular spermatozoa treated with ICSI were randomly assigned into two groups. In the first group, spermatozoa used for injection were selected using the modified HOS test, while in the second group spermatozoa were selected on the basis of their morphology. Results: The fertilization rate was significantly higher in the HOS test group (43.6%) compared with the no-HOS test group (28.2%) [odds ratio (OR) 2.494; 95% confidence interval (CI) 1.606-3.872]. The pregnancy and ongoing pregnancy rates were also higher in the HOS test group (27.3% versus 20.5%) compared with the no-HOS test group (5.7% versus 2.9%) (OR 6.188, 95% CI 1.282-29.860; and OR 8.743, 95% CI 1.050-72.783, respectively). Conclusions: The use of the modified HOS test for the selection of viable sperm from among immotile testicular spermatozoa for ICSI results in higher fertilization, pregnancy and ongoing pregnancy rates compared with morphological selection. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Authors & Co-Authors
Sallam, Hassan Nooman
Egypt, Alexandria
Faculty of Medicine
Egypt, Alexandria
Alexandria Fertility Center
Farrag, A.
Egypt, Alexandria
Faculty of Medicine
Egypt, Alexandria
Alexandria Fertility Center
Agameya, Abdel Fattah
Egypt, Alexandria
Faculty of Medicine
Egypt, Alexandria
Alexandria Fertility Center
El Garem, Yehia
Egypt, Alexandria
Faculty of Medicine
Egypt, Alexandria
Alexandria Fertility Center
Ezzeldin, Fathy
Egypt, Alexandria
Alexandria Fertility Center
Statistics
Citations: 57
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1093/humrep/dei249
ISSN:
02681161
Research Areas
Environmental
Maternal And Child Health
Sexual And Reproductive Health
Study Design
Quasi Experimental Study
Case-Control Study