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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Regulation of Hedgehog Signaling in Chicken Embryonic Stem Cells Differentiation Into Male Germ Cells (Gallus)
Journal of Cellular Biochemistry, Volume 118, No. 6, Year 2017
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Description
The study aims to analyze the key signaling pathways in regulating the process of embryonic stem cells (ESCs) differentiation into spermatogonial stem cells (SSCs). Based on RNA Sequencing result, we further explored the specific regulating mechanisms of Hedgehog (HH) signaling in this process. HH signaling was found to be a crucial signaling pathway participating in the differentiation process of ESCs to SSCs. In Retinoic acid (RA) induced in vitro differentiation assay, the expression of two germ cell marker genes, integrin α6, and integrin β1, was observed to significantly increase, while it decreased dramatically when IHH was knocked down. Fluorescence activated cell sorting analysis showed that the proportion of integrin α6+ and integrin β1+ cells in the RA group was significantly higher than that in the RA + siRNA- Indian Hedgehog (IHH) group. In in vivo situations, siRNA-IHH could stably express in fertilized chicken embryos and significantly down-regulate the IHH expression. With real-time quantitative PCR and western blot, we identified that integrin α6 and integrin β1 expression was significantly suppressed along with IHH inhibition in vivo. We concluded that Hedgehog signaling pathway positively regulates the differentiation of ESCs to male germ cells through signal transduction by IHH. J. Cell. Biochem. 118: 1379–1386, 2017. © 2016 Wiley Periodicals, Inc.
Authors & Co-Authors
Chen, Hao
China, Suzhou
The First Affiliated Hospital of Soochow University
Zuo, Qishenng Sheng
China, Yangzhou
Yangzhou University
Wang, Yinjie
China, Yangzhou
Yangzhou University
Ahmed, Mahmoud F.
Egypt, Ismailia
Faculty of Veterinary Medicine
Jin, Kai
China, Yangzhou
Yangzhou University
Song, Jiuzhou
United States, College Park
University of Maryland, College Park
Zhang, Yani
China, Yangzhou
Yangzhou University
Li, Bichun
China, Yangzhou
Yangzhou University
Statistics
Citations: 13
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1002/jcb.25796
ISSN:
07302312
e-ISSN:
10974644
Research Areas
Cancer
Study Approach
Quantitative
Participants Gender
Male