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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA)
Parasitology Research, Volume 113, No. 4, Year 2014
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Description
To know if alkaline phosphatase (AP) from schistosomes other than Schistosoma mansoni can be used as diagnostic marker for schistosomiasis in alkaline phosphatase immunocapture assay (APIA), we comparatively tested n-butanol extracts of adult worm membranes from a Venezuelan (JL) strain of S. mansoni (Ven/AWBE/Sm); a Cameroonian (EDEN) strain of Schistosoma intercalatum (Cam/AWBE/Si) and a Yemeni strain of Schistosoma haematobium (Yem/AWBE/Sh). APIA was evaluated with sera of patients from Venezuela, Senegal, and Gabon infected with S. mansoni, from Gabon infected with S. intercalatum or S. haematobium, from Chine infected with Schistosoma japonicum and from Cambodian patients infected with Schistosoma mekongi. Results indicate that 92.5 % (37/40) of Venezuela sera, 75 % (15/20) of Senegal sera, 39.5 % (17/43) of S. haematobium sera, and 19.2 % (5/26) S. intercalatum sera were APIA-positive with the Ven/AWBE/Sm preparation. APIA with the Cam/AWBE/Si preparation showed that 53.8 % of S. intercalatum-positive sera had anti-AP antibodies, and 51.2% S. haematobium-positive sera cross-immunocapturing the S. intercalatum AP. APIA performed with Yem/AWBE/Sh showed that 55.8% S. haematobium sera were positive. Only two out of nine S. japonicum sera were APIA-positive with the Ven/AWBE/Sm and Cam/AWBE/Si, and no reaction was observed with Cambodian S. mekongi-positive sera. AP activity was shown to be present in all the schistosome species/strains studied. The use of APIA as a tool to explore the APs antigenicity and the presence of Schistosoma sp. infections through the detection of anti-Schistosoma sp. AP antibodies in a host, allowed us to demonstrate the antigenicity of APs of S. mansoni, S. intercalatum, and S. haematobium. © 2014 Springer-Verlag.
Authors & Co-Authors
Cesari, Italo M.
Venezuela, Caracas
Instituto Venezolano de Investigaciones Cientificas, Centro de Microbiologia y Biologia Celular
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
Ballen, Diana E.
Venezuela, Caracas
Instituto Venezolano de Investigaciones Cientificas, Centro de Microbiologia y Biologia Celular
Mendoza, L.
Venezuela, Caracas
Instituto Venezolano de Investigaciones Cientificas, Centro de Microbiologia y Biologia Celular
Ferrer, Alain
France, Tours
U.f.r. de Médecine
Pointier, Jean Pierre
France, Perpignan
Université de Perpignan Via Domitia
Kombila, Maryvonne Y.
Gabon, Libreville
Faculte de Medecine et Des Sciences de la Sante
Richard-Lenoble, Dominique
France, Tours
U.f.r. de Médecine
Thèron, André É.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 8
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1007/s00436-014-3780-5
ISSN:
09320113
e-ISSN:
14321955
Research Areas
Infectious Diseases
Study Locations
Gabon
Senegal