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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of single nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in some goat breeds in tropical and temperate climates
Molecular Biology Reports, Volume 40, No. 7, Year 2013
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Description
The agouti-signaling protein (ASIP) plays a major role in mammalian pigmentation as an antagonist to melanocortin-1 receptor gene to stimulate pheomelanin synthesis, a major pigment conferring mammalian coat color. We sequenced a 352 bp fragment of ASIP gene spanning part of exon 2 and part of intron 2 in 215 animals representing six goat breeds from Nigeria and the United States: West African Dwarf, predominantly black; Red Sokoto, mostly red; and Sahel, mostly white from Nigeria; black and white Alpine, brown and white Spanish and white Saanen from the US. Twenty haplotypes from nine mutations representing three intronic, one silent and five missense (p.S19R, p.N35K, p.L36V, p.M42L and p.L45W) mutations were identified in Nigerian goats. Approximately 89 % of Nigerian goats carry haplotype 1 (TGCCATCCG) which seems to be the wild type configuration of mutations in this region of the gene. Although we found no association between these polymorphisms in the ASIP gene and coat color in Nigerian goats, in-silico functional analysis predicts putative deleterious functional impact of the p.L45W mutation on the basic amino-terminal domain of ASIP. In the American goats, two intronic mutations, g.293G>A and g.327C>A, were identified in the Alpine breed, although the g.293G>A mutation is common to American and Nigerian goat populations. All Sannen and Sahel goats in this study belong to haplotypes 1 of both populations which seem to be the wild-type composite ASIP haplotype. Overall, there was no clear association of this portion of the ASIP gene interrogated in this study with coat color variation. Therefore, additional genomic analyses of promoter sequence, the entire coding and non-coding regions of the ASIP gene will be required to obtain a definite conclusion. © 2013 Springer Science+Business Media Dordrecht.
Authors & Co-Authors
Adefenwa, Mufliat A.
Nigeria, Lagos
University of Lagos
United States, Ithaca
Cornell University
Peters, Sunday Olusola
United States, Mount Berry
Berry College
United States, Ithaca
Cornell University
Agaviezor, Brilliant Ogagaoghene
Nigeria, Port Harcourt
University of Port Harcourt
United States, Ithaca
Cornell University
Wheto, Mathew Y.
Nigeria, Abeokuta
Federal University of Agriculture, Abeokuta
Adekoya, Khalid Olajide
Nigeria, Lagos
University of Lagos
Okpeku, M.
Nigeria, Amassoma
Niger Delta University
Oboh, Bola Olufunmilayo
Nigeria, Lagos
University of Lagos
Williams, Gabriel O.
Nigeria, Lagos
University of Lagos
Adebambo, Olufunmilayo Ayoka
Nigeria, Abeokuta
Federal University of Agriculture, Abeokuta
Singh, Mahipal
United States, Fort Valley
Fort Valley State University
Thomas, Bolaji Nun
United States, Rochester
Rochester Institute of Technology
De Donato-Capote, Marcos
United States, Ithaca
Cornell University
Imumorin, Ikhide G.
United States, Ithaca
Cornell University
Statistics
Citations: 16
Authors: 13
Affiliations: 8
Identifiers
Doi:
10.1007/s11033-013-2535-1
ISSN:
03014851
e-ISSN:
15734978
Research Areas
Cancer
Genetics And Genomics
Study Locations
Nigeria