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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Identification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestations
Parasitology Research, Volume 106, No. 2, Year 2010
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Description
The cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant Rhipicephalus microplus Bm86 antigen has been shown to protect cattle against tick infestations. However, variable efficacy of Bm86-based vaccines against geographic tick strains has encouraged the research for additional tick-protective antigens. Herein, we describe the analysis of R. microplus glutathione-S transferase, ubiquitin (UBQ), selenoprotein W, elongation factor-1 alpha, and subolesin (SUB) complementary DNAs (cDNAs) by RNA interference (RNAi) in R. microplus and Rhipicephalus annulatus. Candidate protective antigens were selected for vaccination experiments based on the effect of gene knockdown on tick mortality, feeding, and fertility. Two cDNA clones encoding for UBQ and SUB were used for cattle vaccination and infestation with R. microplus and R. annulatus. Control groups were immunized with recombinant Bm86 or adjuvant/saline. The highest vaccine efficacy for the control of tick infestations was obtained for Bm86. Although with low immunogenic response, the results with the SUB vaccine encourage further investigations on the use of recombinant subolesin alone or in combination with other antigens for the control of cattle tick infestations. The UBQ peptide showed low immunogenicity, and the results of the vaccination trial were inconclusive to assess the protective efficacy of this antigen. These experiments showed that RNAi could be used for the selection of candidate tick-protective antigens. However, vaccination trials are necessary to evaluate the effect of recombinant antigens in the control of tick infestations, a process that requires efficient recombinant protein production and formulation systems. © The Author(s) 2009.
Authors & Co-Authors
Almazán, Consuelo
Mexico, Ciudad Victoria
Universidad Autónoma de Tamaulipas
Lagunes, Rodolfo
Mexico, Mexico
Universidad Nacional Autónoma de México
Villar, Margarita
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Canales, Mario
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Rosario-Cruz, Rodrigo
Mexico, Cuernavaca
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria
Jongejan, Frans
Netherlands, Utrecht
Universiteit Utrecht
South Africa, Pretoria
University of Pretoria
de la Fuente, José Luis
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
United States, Stillwater
Oklahoma State University
Statistics
Citations: 118
Authors: 7
Affiliations: 7
Identifiers
Doi:
10.1007/s00436-009-1689-1
ISSN:
09320113
Research Areas
Genetics And Genomics
Sexual And Reproductive Health