Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Differential profiles and inhibitory effect on rotavirus vaccines of nonantibody components in breast milk from mothers in developing and developed countries
Pediatric Infectious Disease Journal, Volume 32, No. 8, Year 2013
Notification
URL copied to clipboard!
Description
BACKGROUND: Live oral rotavirus vaccines have been less immunogenic and efficacious for children of developing countries than for those in middle income and industrialized countries, and the basis for these differences is not fully understood. Recently, we demonstrated that breastmilk from mothers in India had significantly higher IgA and neutralizing activity against rotavirus that could reduce the effective titer of rotavirus vaccines reaching the gut when compared with that from mothers in the United States. We extended our study to understand the specific contribution of those nonantibody components in breastmilk to the neutralizing activity against rotavirus vaccine we observed. METHODS: Breastmilk samples were collected from mothers of breast-feeding infants aged between 4 and 29 weeks (ie, vaccine eligible age) in India (N = 40), South Africa (N = 50) and the United States (N = 51). We examined breastmilk for lactoferrin, lactadherin, rotavirus-specific IgA and neutralizing activity against 3 rotavirus vaccine strains (Rotarix, RotaTeq G1 and 116E) using enzyme immunoassays, a plaque reduction assay or a microneutralization assay. RESULTS: We observed higher levels of lactoferrin, lactadherin, IgA and neutralizing activity in breastmilk specimens from Indian and South African women than those from American women. We demonstrated positive associations between levels of lactoferrin or IgA and neutralizing activity in Indian and South African specimens, but not in American specimens. We demonstrated that the inhibitory effect of lactoferrin was dose-or species-dependent, as evidenced by greater reduction in titer of Rotarix and 116E by human lactoferrin. Lactadherin also exhibited inhibitory activity to rotavirus vaccines but appeared to be less effective. CONCLUSIONS: The lower immunogenicity and efficacy of rotavirus vaccines in developing countries could be explained, in part, by synergistic inhibitory effect of high levels of antibody and nonantibody components in breastmilk consumed by infants at the time of immunization. Therefore, there is a need for alternative rotavirus vaccine strategies in breast-feeding populations. Copyright © 2013 Lippincott Williams &Wilkins.
Authors & Co-Authors
Moon, Sungsil
United States, Atlanta
Centers for Disease Control and Prevention
Tate, Jacqueline E.
United States, Atlanta
Centers for Disease Control and Prevention
Ray, P.
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Dennehy, Penelope Hill
United States, Providence
Rhode Island Hospital
Archary, Derseree
South Africa, Durban
University of Kwazulu-natal
Coutsoudis, Anna
South Africa, Durban
University of Kwazulu-natal
Bland, Ruth M.
South Africa, Durban
University of Kwazulu-natal
United Kingdom, Glasgow
University of Glasgow
Newell, Marie Louise
South Africa, Durban
University of Kwazulu-natal
United Kingdom, London
Ucl Great Ormond Street Institute of Child Health
Glass, Roger I.M.
United States, Bethesda
National Institutes of Health Nih
Parashar, Umesh D.
United States, Atlanta
Centers for Disease Control and Prevention
Jiang, Baoming
United States, Atlanta
Centers for Disease Control and Prevention
Statistics
Citations: 57
Authors: 11
Affiliations: 7
Identifiers
Doi:
10.1097/INF.0b013e318290646d
ISSN:
08913668
e-ISSN:
15320987
Research Areas
Health System And Policy
Maternal And Child Health
Study Locations
South Africa
Participants Gender
Female