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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Use of alternate coreceptors on primary cells by two HIV-1 isolates
Virology, Volume 339, No. 1, Year 2005
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Description
Two HIV-1 isolates (CM4 and CM9) able to use alternate HIV-1 coreceptors on transfected cell lines were tested for their sensitivity to inhibitors of HIV-1 entry on primary cells. CM4 was able to use CCR5 and Bob/GPR15 efficiently in transfected cells. The R5 isolate grew in Δ32/Δ32 CCR5 PBMC in the absence or presence of AMD3100, a CXCR4-specific inhibitor, indicating that it uses a receptor other than CCR5 or CXCR4 on primary cells. It was insensitive to the CCR5 entry inhibitors RANTES and PRO140, but was partially inhibited by vMIP-1, a chemokine that binds CCR3, CCR8, GPR15 and CXCR6. The coreceptor used by this isolate on primary cells is currently unknown. CM9 used CCR5, CXCR4, Bob/GPR15, CXCR6, CCR3, and CCR8 on transfected cells and was able to replicate in the absence or presence of AMD3100 in Δ32/Δ32 CCR5 PBMC. It was insensitive to eotaxin, vMIP-1 and I309 when tested individually, but was inhibited completely when vMIP-1 or I309 was combined with AMD3100. Both I309 and vMIP-1 bind CCR8, strongly suggesting that this isolate can use CCR8 on primary cells. Collectively, these data suggest that some HIV-1 isolates can use alternate coreceptors on primary cells, which may have implications for strategies that aim to block viral entry. © 2005 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Cilliers, Tonie
South Africa, Johannesburg
National Institute for Communicable Diseases
Willey, Samantha
United States, Worcester
University of Massachusetts Chan Medical School
Sullivan, W. Mathew
United States, Worcester
University of Massachusetts Chan Medical School
Patience, Trudy
South Africa, Johannesburg
National Institute for Communicable Diseases
Pugach, Pavel
United States, New York
Weill Cornell Medicine
Coetzer, Mia E.
South Africa, Johannesburg
National Institute for Communicable Diseases
Papathanasopoulos, Maria
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Clinical Medicine
Moore, John P.
United States, New York
Weill Cornell Medicine
Trkola, Alexandra
Switzerland, Zurich
Universitatsspital Zurich
Clapham, Paul R.
United States, Worcester
University of Massachusetts Chan Medical School
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 53
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1016/j.virol.2005.05.027
ISSN:
00426822
Research Areas
Infectious Diseases