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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
HIV-1 specific IgA detected in vaginal secretions of HIV uninfected women participating in a microbicide trial in Southern Africa are primarily directed toward gp120 and gp140 specificities
PLoS ONE, Volume 9, No. 7, Article e101863, Year 2014
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Description
Background: Many participants in microbicide trials remain uninfected despite ongoing exposure to HIV-1. Determining the emergence and nature of mucosal HIV-specific immune responses in such women is important, since these responses may contribute to protection and could provide insight for the rational design of HIV-1 vaccines. Methods and Findings: We first conducted a pilot study to compare three sampling devices (Dacron swabs, flocked nylon swabs and Merocel sponges) for detection of HIV-1-specific IgG and IgA antibodies in vaginal secretions. IgG antibodies from HIV-1-positive women reacted broadly across the full panel of eight HIV-1 envelope (Env) antigens tested, whereas IgA antibodies only reacted to the gp41 subunit. No Env-reactive antibodies were detected in the HIV-negative women. The three sampling devices yielded equal HIV-1-specific antibody titers, as well as total IgG and IgA concentrations. We then tested vaginal Dacron swabs archived from 57 HIV seronegative women who participated in a microbicide efficacy trial in Southern Africa (HPTN 035). We detected vaginal IgA antibodies directed at HIV-1 Env gp120/gp140 in six of these women, and at gp41 in another three women, but did not detect Env-specific IgG antibodies in any women. Conclusion: Vaginal secretions of HIV-1 infected women contained IgG reactivity to a broad range of Env antigens and IgA reactivity to gp41. In contrast, Env-binding antibodies in the vaginal secretions of HIV-1 uninfected women participating in the microbicide trial were restricted to the IgA subtype and were mostly directed at HIV-1 gp120/gp140. © 2014 Seaton et al.
Authors & Co-Authors
Seaton, Kelly E.
United States, Durham
Duke University School of Medicine
Ballweber, Lamar M.
United States, Seattle
University of Washington
Lan, Audrey
United States, Durham
Duke University School of Medicine
Donathan, Michele
United States, Durham
Duke University School of Medicine
Hughes, Sean M.
United States, Seattle
University of Washington
Vojtech, Lucia N.
United States, Seattle
University of Washington
Moody, Michael Anthony
United States, Durham
Duke University School of Medicine
Liao, Huaxin
United States, Durham
Duke University School of Medicine
Haynes, Barton F.
United States, Durham
Duke University School of Medicine
Galloway, Christine G.
United States, Seattle
Fred Hutchinson Cancer Research Center
Richardson, Barbra Ann
United States, Seattle
Fred Hutchinson Cancer Research Center
United States, Seattle
University of Washington
Abdool Karim, Salim S.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
United States, New York
Columbia University
Dezzutti, Charlene S.
United States, Pittsburgh
University of Pittsburgh School of Medicine
McElrath, Margaret Juliana
United States, Seattle
Fred Hutchinson Cancer Research Center
United States, Seattle
University of Washington
Tomaras, Georgia D.
United States, Durham
Duke University School of Medicine
Hladik, Florian
United States, Seattle
University of Washington
United States, Seattle
Fred Hutchinson Cancer Research Center
Statistics
Citations: 36
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0101863
e-ISSN:
19326203
Research Areas
Infectious Diseases
Participants Gender
Female