Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Comparison of LC-UV, LC-ELSD and LC-MS methods for the determination of sesquiterpenoids in various species of artemisia
Chromatographia, Volume 70, No. 5-6, Year 2009
Notification
URL copied to clipboard!
Description
Simple and specific analytical methods for the quantitative determination of sesquiterpenoids from various species of Artemisia plant samples were developed. By LC-UV, LC-ELSD, the separation was achieved by reversed-phase chromatography on a C18 column with water and acetonitrile both containing 0.025% trifluoroacetic acid as the mobile phase. In the LC-MS system, trifluoroacetic acid was replaced by 0.1% formic acid. The wavelength used for quantification of sesquiterpenoids with a diode array detector was 205 nm. The limits of detection by LC-MS was found to be 5, 10, 25, 50, 50 ng mL -1. The limits of detection by LC-UV and LC-ELSD were found to be 5.0, 3.0, 100, 100, 7.5 μg mL-1, by LC-UV and 50, 25, 30, 100 and 75 μg mL-1 by LC-ELSD. LC-mass spectrometry coupled with electrospray ionization (ESI) interface is described for the identification and quantification of sesquiterpenoids in various plant samples. This method involved the use of the [M + H]+ ions of sesquiterpenoids in the positive ion mode with extractive ion monitoring. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH.
Authors & Co-Authors
Avula, Bharathi
United States, University
University of Mississippi, Research Institute Pharmaceutical Science
Wang, Yanhong
United States, University
University of Mississippi, Research Institute Pharmaceutical Science
Smillie, Troy J.
United States, University
University of Mississippi, Research Institute Pharmaceutical Science
Mabusela, Wilfred T.
South Africa, Bellville
University of the Western Cape
Vincent, Leszek
United States, Columbia
University of Missouri
Weitz, Frans M.
South Africa, Bellville
University of the Western Cape
Khan, Ikhlas A.
United States, University
University of Mississippi, Research Institute Pharmaceutical Science
United States, University
University of Mississippi
Statistics
Citations: 20
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1365/s10337-009-1237-2
ISSN:
00095893
e-ISSN:
16121112
Research Areas
Environmental
Study Approach
Quantitative