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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
MHC class I bound to an immunodominant Theileria parva epitope demonstrates unconventional presentation to T cell receptors
PLoS Pathogens, Volume 6, No. 10, Article e1001149, Year 2010
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Description
T cell receptor (TCR) recognition of peptide-MHC class I (pMHC) complexes is a crucial event in the adaptive immune response to pathogens. Peptide epitopes often display a strong dominance hierarchy, resulting in focusing of the response on a limited number of the most dominant epitopes. Such T cell responses may be additionally restricted by particular MHC alleles in preference to others. We have studied this poorly understood phenomenon using Theileria parva, a protozoan parasite that causes an often fatal lymphoproliferative disease in cattle. Despite its antigenic complexity, CD8+ T cell responses induced by infection with the parasite show profound immunodominance, as exemplified by the Tp1214-224 epitope presented by the common and functionally important MHC class I allele N*01301. We present a high-resolution crystal structure of this pMHC complex, demonstrating that the peptide is presented in a distinctive raised conformation. Functional studies using CD8+ T cell clones show that this impacts significantly on TCR recognition. The unconventional structure is generated by a hydrophobic ridge within the MHC peptide binding groove, found in a set of cattle MHC alleles. Extremely rare in all other species, this feature is seen in a small group of mouse MHC class I molecules. The data generated in this analysis contribute to our understanding of the structural basis for T cell-dependent immune responses, providing insight into what determines a highly immunogenic p-MHC complex, and hence can be of value in prediction of antigenic epitopes and vaccine design. © 2010 Macdonald et al.
Authors & Co-Authors
Connelley, Timothy K.
United Kingdom, Edinburgh
The University of Edinburgh
MacHugh, Niall D.
United Kingdom, Edinburgh
The University of Edinburgh
Graham, Simon P.
United Kingdom, Addlestone
Animal and Plant Health Agency
Jones, Edith Yvonne
United Kingdom, Oxford
University of Oxford
Morrison, William Ivan
United Kingdom, Edinburgh
The University of Edinburgh
Flower, Darren R.
United Kingdom, Oxford
The Jenner Institute
Ellis, Shirley A.
United Kingdom, Surrey
The Pirbright Institute
Statistics
Citations: 46
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1371/journal.ppat.1001149
ISSN:
15537374
Research Areas
Genetics And Genomics