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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Gold nanoparticle based Tuberculosis immunochromatographic assay: The quantitative ESE Quanti analysis of the intensity of test and control lines
Biosensors and Bioelectronics, Volume 54, Year 2014
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Description
A rapid dual channel lateral flow assay for the detection of Mycobacterium Tuberculosis antibodies (MTB 38. kDa monoclonal antibody) in human blood was developed. The MTB 6-14-38. kDa fusion antigen and anti-Protein A were used as the capture proteins for test and control lines respectively. Protein A labeled 40. nm gold nanoparticles were used as the detection conjugate. Whole blood and serum were spiked with MTB 38. kDa monoclonal antibody to make a positive sample model. The developed lateral flow was used to test MTB 38. kDa monoclonal antibody, and a detection limit of 5. ng/ml was used as a cut-off concentration of the analytes. The effect of the analyte concentration on the MTB lateral flow assay was studied using the variation of the intensity obtained from a ESE Quanti reader. There was a direct correlation between the analyte (MTB 38. kDa monoclonal antibody) concentration and the intensity of the test line. The intensity increased with an increase in the concentration of MTB 38. kDa monoclonal antibody, while in contrast, an increase in analyte concentration decreased the intensity of the control line. © 2013 Elsevier B.V.
Authors & Co-Authors
Mdluli, Phumlane Selby
South Africa, Randburg
Mintek
Tetyana, Phumlani
South Africa, Randburg
Mintek
Sosibo, Ndabenhle M.
South Africa, Randburg
Mintek
van der Walt, Hendriëtte
South Africa, Randburg
Mintek
Mlambo, Mbuso
South Africa, Randburg
Mintek
Skepu, Amanda
South Africa, Randburg
Mintek
Tshikhudo, Robert T.
South Africa, Randburg
Mintek
Statistics
Citations: 26
Authors: 7
Affiliations: 1
Identifiers
Doi:
10.1016/j.bios.2013.10.019
ISSN:
09565663
e-ISSN:
18734235
Study Approach
Quantitative