Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Changes in P2Y
2
receptor localization on adrenaline- and noradrenaline-containing chromaffin cells in the rat adrenal gland during development and aging
International Journal of Developmental Neuroscience, Volume 23, No. 7, Year 2005
Notification
URL copied to clipboard!
Description
Using immunohistochemistry, the occurrence and age-related changes of the P2Y2 receptor was investigated in the adrenal gland of rat at different ages, ranging from embryonic day E16 to 22 months. Immunoreactivity for the P2Y2 receptor was present in chromaffin cells and nerve fibres at all ages examined. Double labeling with the antibody against phenyl ethanolamine-N-methyltransferase, which marks adrenaline-producing chromaffin cells, revealed that only a few of the P2Y2-immunoreactive cells were adrenaline producing at embryonic day E16, the vast majority being noradrenaline-containing cells. However, immunoreactivity for adrenaline-containing cells in the P2Y2 receptor-labeled chromaffin cells increased with increasing age and at 1 week post-natal almost all chromaffin cells were positive for both P2Y2 and phenyl ethanolamine-N-methyltransferase, while noradrenaline-containing cells were minimal. At 2 weeks, there was a dramatic drop in P2Y2-immunoreactive chromaffin cells and this was maintained in adult rats, noradrenaline- containing cells dominating. In the aging rat adrenals, P2Y2 receptor-immunoreactivity was localized in subpopulations of both adrenaline and noradrenaline-producing cells. Intrinsic neurones were also visible that were positively labeled with the P2Y2 receptor antibody in the adrenals of both adult and aging rats. P2Y2-immunoreactive nerve fibres formed a plexus around the adrenal cortical cells of zona glomerulosa in the post-natal, but not in adult or aging rats. In conclusion, this study suggests that ATP, acting through P2Y2 receptors, may influence the phenotypic expression of chromaffin cells during the development and aging of the rat adrenal gland. However, during early development, when the chromaffin cells are actively dividing and during aging, when the adrenal medullary cells are known to show hyperplastic lesions, ATP acting through P2Y2 receptors may be involved in other physiological activities, such as proliferation and/or differentiation of the chromaffin cells associated with their adrenaline or noradrenaline phenotype. © 2005 ISDN. Published by Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Afework, Mekbeb
United Kingdom, London
Ucl Medical School
Ethiopia, Addis Ababa
School of Medicine
Burnstock, Geoffrey
United Kingdom, London
Ucl Medical School
Statistics
Citations: 12
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijdevneu.2005.07.004
ISSN:
07365748