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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Nanostructural analysis by atomic force microscopy followed by light microscopy on the same archival slide
Microscopy Research and Technique, Volume 72, No. 7, Year 2009
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Description
Integrated information on ultrastructural surface texture and chemistry increasingly plays a role in the biomedical sciences. Light microscopy provides access to biochemical data by the application of dyes. Ultrastructural representation of the surface structure of tissues, cells, or macromolecules can be obtained by scanning electron microscopy (SEM). However, SEM often requires gold or coal coating of biological samples, which makes a combined examination by light microscopy and SEM difficult. Conventional histochemical staining methods are not easily applicable to biological material subsequent to such treatment. Atomic force microscopy (AFM) gives access to surface textures down to ultrastructural dimensions without previous coating of the sample. A combination of AFM with conventional histochemical staining protocols for light microscopy on a single slide is therefore presented. Unstained cores were examined using AFM (tapping mode) and subsequently stained histochemically. The images obtained by AFM were compared with the results of histochemistry. AFM technology did not interfere with any of the histochemical staining protocols. Ultrastructurally analyzed regions could be identified in light microscopy and histochemical properties of ultrastructurally determined regions could be seen. AFM-generated ultrastructural information with subsequent staining gives way to novel findings in the biomedical sciences. © 2009 Wiley-Liss, Inc.
Authors & Co-Authors
Wagner, Mathias
Germany, Homburg
Universitätsklinikum Des Saarlandes Medizinische Fakultät Der Universität Des Saarlandes
Kaehler, Dirk
Germany, Itzehoe
Fraunhofer Institute for Silicon Technology Isit
Germany, Bochum
Ruhr-universitat Bochum
Anhenn, Olaf
Germany, Essen
Medizinischen Fakultät
Betz, Thomas
Germany, Saarbrucken
Universität Des Saarlandes
Awad, Sally
Egypt, Mansoura
Mansoura University
Shamaa, Ali A.
Egypt, Minya
Minia University
Theegarten, Dirk
Germany, Essen
Medizinischen Fakultät
Linder, Roland
Germany, Lubeck
Universität zu Lübeck
Statistics
Citations: 8
Authors: 8
Affiliations: 8
Identifiers
Doi:
10.1002/jemt.20691
ISSN:
1059910X
e-ISSN:
10970029
Research Areas
Health System And Policy