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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
The multifunctional polydnavirus TnBVANK1 protein: Impact on host apoptotic pathway
Scientific Reports, Volume 7, No. 1, Article 11775, Year 2017
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Description
Toxoneuron nigriceps (Hymenoptera, Braconidae) is an endophagous parasitoid of the larval stages of the tobacco budworm, Heliothis virescens (Lepidoptera, Noctuidae). The bracovirus associated with this wasp (TnBV) is currently being studied. Several genes expressed in parasitised host larvae have been isolated and their possible roles partly elucidated. TnBVank1 encodes an ankyrin motif protein similar to insect and mammalian IκB, an inhibitor of the transcription nuclear factor κB (NF-κB). Here we show that, when TnBVank1 was stably expressed in polyclonal Drosophila S2 cells, apoptosis is induced. Furthermore, we observed the same effects in haemocytes of H. virescens larvae, after TnBVank1 in vivo transient transfection, and in haemocytes of parasitised larvae. Coimmunoprecipitation experiments showed that TnBVANK1 binds to ALG-2 interacting protein X (Alix/AIP1), an interactor of apoptosis-linked gene protein 2 (ALG-2). Using double-immunofluorescence labeling, we observed the potential colocalization of TnBVANK1 and Alix proteins in the cytoplasm of polyclonal S2 cells. When Alix was silenced by RNA interference, TnBVANK1 was no longer able to cause apoptosis in both S2 cells and H. virescens haemocytes. Collectively, these results indicate that TnBVANK1 induces apoptosis by interacting with Alix, suggesting a role of TnBVANK1 in the suppression of host immune response observed after parasitisation by T. nigriceps. © 2017 The Author(s).
Authors & Co-Authors
Salvia, Rosanna
Italy, Potenza
Università Degli Studi Della Basilicata
Amoresano, Angela
Italy, Naples
Università Degli Studi Di Napoli Federico Ii
Scieuzo, Carmen
Italy, Potenza
Università Degli Studi Della Basilicata
Nardiello, Marisa
Italy, Potenza
Università Degli Studi Della Basilicata
Laurenzana, Ilaria
Italy, Rionero in Vulture
Irccs-crob, Referral Cancer Center of Basilicata
Bufo, Sabino Aurelio
Italy, Potenza
Università Degli Studi Della Basilicata
Vinson, S. Bradleigh
United States, College Station
Texas A&m University
Carmosino, Monica
Italy, Potenza
Università Degli Studi Della Basilicata
Vogel, Heiko
Germany, Jena
Max Planck Institute for Chemical Ecology
Falabella, Patrizia
Italy, Potenza
Università Degli Studi Della Basilicata
Statistics
Citations: 21
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1038/s41598-017-11939-x
ISSN:
20452322
Research Areas
Cancer
Genetics And Genomics
Substance Abuse