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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in a rat liver cell line
Toxicology and Applied Pharmacology, Volume 253, No. 1, Year 2011
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Description
Dynamic analysis of the uptake and metabolism of polycyclic aromatic hydrocarbons (PAHs) and their metabolites within live cells in real time has the potential to provide novel insights into genotoxic and non-genotoxic mechanisms of cellular injury caused by PAHs. The present work, combining the use of metabolite spectra generated from metabolite standards using multiphoton spectral analysis and an "advanced unmixing process", identifies and quantifies the uptake, partitioning, and metabolite formation of one of the most important PAHs (benzo[. a]pyrene, BaP) in viable cultured rat liver cells over a period of 24. h. The application of the advanced unmixing process resulted in the simultaneous identification of 8 metabolites in live cells at any single time. The accuracy of this unmixing process was verified using specific microsomal epoxide hydrolase inhibitors, glucuronidation and sulfation inhibitors as well as several mixtures of metabolite standards. Our findings prove that the two-photon microscopy imaging surpasses the conventional fluorescence imaging techniques and the unmixing process is a mathematical technique that seems applicable to the analysis of BaP metabolites in living cells especially for analysis of changes of the ultimate carcinogen benzo[. a]pyrene-r-7,. t-8-dihydrodiol-t-9,10-epoxide. Therefore, the combination of the two-photon acquisition with the unmixing process should provide important insights into the cellular and molecular mechanisms by which BaP and other PAHs alter cellular homeostasis. © 2011 Elsevier Inc.
Authors & Co-Authors
Barhoumi, Rola
Unknown Affiliation
Mouneimne, Youssef
Unknown Affiliation
Ramos, Ernesto
Unknown Affiliation
Morisseau, Christophe H.P.
Unknown Affiliation
Hammock, Bruce D.
Unknown Affiliation
Safe, Stephen H.
Unknown Affiliation
Parrish, Alan R.
Unknown Affiliation
Burghardt, Robert C.
Unknown Affiliation
Statistics
Citations: 15
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1016/j.taap.2011.03.009
ISSN:
0041008X
e-ISSN:
10960333
Research Areas
Violence And Injury