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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
In vitro corm production of Gladiolus dalenii and G. tristis
Plant Cell, Tissue and Organ Culture, Volume 31, No. 2, Year 1992
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Description
The ability of the summer flowering Gladiolus dalenii Van Geel and the winter flowering G. tristis L. to form corms in vitro was investigated. G. dalenii spontaneously formed corms on a shoot induction medium consisting of the basal medium of Murashige & Skoog (1962) with up to 2.0 mg l-1 benzyladenine (BA), 3% sucrose and solidified with 2 g l-1 Gelrite®. The effect of different BA and sucrose concentrations as well as different temperatures on in vitro corm production of G. tristis was further investigated. The best production of shoots per explant was achieved on a medium containing 0.5 to 1.0 mg l-1 BA, sucrose concentrations of 6 to 9% and cultured at 15°C. The best corm production was achieved at the same temperature and with the same medium with the exception that BA was omitted from the medium. To test the effect of the osmotic potential on the formation of shoots and corms, sucrose was substituted by mannitol at various concentrations. Sucrose proved to be essential for both shoot and corm production and the use of mannitol had no beneficial effect. © 1992 Kluwer Academic Publishers.
Authors & Co-Authors
de Bruyn, Marienne H.
South Africa, Pretoria
Vegetable and Ornamental Plant Research Institute
Ferreira, David I.
South Africa, Pretoria
Plant Biotechnology Research Centre
Statistics
Citations: 42
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1007/BF00037696
ISSN:
01676857
e-ISSN:
15735044