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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Detection of Telomerase in Urine by 3 Methods: Evaluation of Diagnostic Accuracy for Bladder Cancer
Journal of Urology, Volume 178, No. 3, Year 2007
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Description
Purpose: New, noninvasive methods are needed for the diagnosis, followup and screening of patients with bladder cancer. Three methods of detecting telomerase were evaluated in this aspect. Materials and Methods: This study included 200 patients diagnosed with bladder carcinoma, 85 with benign bladder lesions and 30 healthy individuals who served as the control group. All underwent serological schistosomiasis antibody assay in serum, urine cytology and estimation of relative telomerase activity by telomeric repeat amplification protocol, human telomerase RNA by reverse transcriptase-polymerase chain reaction and human telomerase reverse transcriptase by real-time reverse transcriptase-polymerase chain reaction in urothelial cells from voided urine. Results: The concordance between the positive rates of telomerase detected by the 3 methods was high (90% to 95%). Results were significantly higher in the malignant group than in the benign and control groups. There was a significant difference among the results of the 3 methods in relation to different clinicopathological factors. Overall the sensitivity of human telomerase reverse transcriptase for detecting bladder cancer was the highest compared to that of human telomerase RNA, relative telomerase activity and urine cytology (96%, 92%, 75% and 75%, respectively). Combinations of telomerase results with urine cytology were not useful except in cases of relative telomerase activity. Conclusions: Detection of human telomerase reverse transcriptase in urine by real-time polymerase chain reaction, followed by human telomerase RNA by reverse transcriptase-polymerase chain reaction, improves sensitivity and specificity for the diagnosis of bladder cancer. However, regarding cost-effectiveness, human telomerase RNA is superior. © 2007 American Urological Association.
Authors & Co-Authors
Eissa, S.
Egypt, Cairo
Faculty of Medicine
Swellam, Menha M.
Egypt, Giza
National Research Centre
Ali-Labib, Randa
Egypt, Cairo
Faculty of Medicine
Mansour, Amal A.
Egypt, Cairo
Faculty of Medicine
El-Malt, Osama A.
Egypt, Giza
Cairo University
Tash, Fathy Mohamed
Egypt, Cairo
Faculty of Medicine
Statistics
Citations: 51
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.juro.2007.05.006
ISSN:
00225347
Research Areas
Cancer
Infectious Diseases
Study Design
Randomised Control Trial