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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of cytokine mRNA in the brains of mice with toxoplasmic encephalitis
Parasite Immunology, Volume 14, No. 4, Year 1992
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Description
Summary C57B1/10 ScSn mice infected with Toxoplasma gondii developed a meningoencephalitis, characterized by areas of tissue destruction and cellular infiltration including foci of neutrophils. Large numbers of cyst stages were found throughout the brain but were not always associated with inflammation. The use of immunocytochemistry to detect glial fibrillary acidic protein, an astrocyte specific marker, showed a widespread astrocyte activation. This was particularly prominent in areas of intense inflammation but cysts were negative for glial fibrillary acidic protein, indicating that astrocytes were not host cells for the bradyzoites. The use of the polymerase chain reaction to assist in the amplification of total brain RNA allowed the characterization of the cytokines being produced locally within the brains of infected animals. β‐ actin transcripts were detected in all of the uninfected and infected mice. In only one of the seven uninfected control mice were other transcripts found. Transcripts for tumour necrosis factor‐α, interleukin‐1α and β, interleukin‐6, macrophage inflammatory protein‐1 and interferon‐γ as well as the CD4 marker were detected in all of the infected mice. However, transcripts for IL‐2 and IL‐4 were not present. Several of the cytokines present are capable of initiating meningeal inflammation and may play a role in the immunopathogensis of toxoplasmic encephalitis. Copyright © 1992, Wiley Blackwell. All rights reserved
Authors & Co-Authors
Hunter, Christopher A.
United Kingdom, Glasgow
University of Glasgow
Roberts, Craig W.
United Kingdom, Glasgow
University of Strathclyde
Murray, Maxwell D.
United Kingdom, Glasgow
University of Glasgow
Alexander, James B.
United Kingdom, Glasgow
University of Strathclyde
Statistics
Citations: 43
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1111/j.1365-3024.1992.tb00015.x
ISSN:
01419838
Research Areas
Cancer