Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains
Veterinary Microbiology, Volume 109, No. 3-4, Year 2005
Notification
URL copied to clipboard!
Description
The discriminatory power of variable number tandem repeat (VNTR) typing based on 16 known loci (12 MIRUs, 3 ETRs and VNTR 3232) was assessed for Mycobacterium bovis strains collected sequentially at the slaughterhouse of N'Djaména, Chad. Of 67 M. bovis strains analyzed, 67% were clustered. In this study, VNTR typing was highly discriminative with an overall allelic diversity (hoa) of 0.922. We defined five loci (ETR A, B, C and MIRU 26, 27) as highly (h > 0.25), two loci (MIRU 4, and VNTR 3232) as moderately (0.11 < h < 0.25) and three loci (MIRU 16, 20, 31) as poorly (0.01 < h < 0.11) discriminative. Six loci (MIRU 2, 10, 23, 24, 39, and 40) showed no polymorphism at all. VNTR typing of the five highly discriminative loci (h = 0.917) proved to be most appropriate for first line typing of M. bovis strains of Chad and superior than spoligotyping (hsp = 0.789). In contrast to Mycobacterium tuberculosis strains, a consensus on VNTR loci needs to be found for M. bovis strains. The selection of a generally agreed set of VNTR loci for molecular discrimination of M. bovis in different geographical settings is discussed. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hilty, Markus
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Diguimbaye, Colette D.
Chad
Laboratoire de Recherches Vétérinaires et Zootechniques de Farcha
Schelling, Esther
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Baggi, Franca
Switzerland, Zurich
National Center for Mycobacteria
Tanner, Marcel
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Zinsstag, Jakob B.
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Statistics
Citations: 80
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.vetmic.2005.05.017
ISSN:
03781135
Study Locations
Chad