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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Whole-transcriptome analysis of Plasmodium falciparum field isolates: Identification of new pathogenicity factors
Journal of Infectious Diseases, Volume 196, No. 11, Year 2007
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Description
Background. Severe malaria and one of its most important pathogenic processes, cerebral malaria, involves the sequestration of parasitized red blood cells (pRBCs) in brain postcapillary veinules. Although the pathogenic mechanisms underlying malaria remain poorly characterized, it has been established that adhesion of pRBCs to endothelial cells (ECs) can result in cell apoptosis, which in turn may lead to disruption of the blood-brain barrier. The nature of the parasite molecules involved in the pathogenesis of severe malaria remains elusive. Methods. Whole-transcriptome profiling of nonapoptogenic versus apoptogenic parasite field isolates obtained from Gabonese children was performed with pan-genomic Plasmodium falciparum DNA microarrays; radiolabeled instead of fluorescent cDNAs were used to improve the sensitivity of signal detection. Results. Our methods allowed the identification of 59 genes putatively associated with the induction of EC apoptosis. Silencing of Plasmodium gene expression with specific double-stranded RNA was performed on 8 selected genes; 5 of these, named "Plasmodium apoptosis-linked pathogenicity factors" (PALPFs), were found to be linked to parasite apoptogenicity. Of these genes, 2 might act via parasite cytoadherence. Conclusion. This is the first attempt to identify genes involved in parasite pathogenic mechanisms against human ECs. The finding of PALPFs illuminates perspectives for novel therapeutic strategies against cerebral complications of malaria. © 2007 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Siau, Anthony
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
Touré-Ndouo, Fousseyni Samba
Gabon, Franceville
Centre International de Recherches Medicales de Franceville
Ouwe-Missi-Oukem-Boyer, Odile N.
Gabon, Franceville
Centre International de Recherches Medicales de Franceville
Cicéron, Liliane
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
Mahmoudi, Nassira
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
Vaquero, Catherine M.
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
Froissard, Patrick
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
Bisvigou, Ulrich Jolhy
Gabon, Franceville
Centre International de Recherches Medicales de Franceville
Bisser, Sylvie
Gabon, Franceville
Centre International de Recherches Medicales de Franceville
Coppeé, Jean Yves
France, Evry
Genopole
Bischoff, Emmanuel
France, Evry
Genopole
David, Peter H.
France, Paris
Institut Pasteur, Paris
Mazier, Dominique
France
Institut National de la Santé et de la Recherche Médicale U511
France, Paris
Sorbonne Université
France, Paris
Hôpital Universitaire Pitié Salpêtrière
Statistics
Citations: 32
Authors: 13
Affiliations: 6
Identifiers
Doi:
10.1086/522012
ISSN:
00221899
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Maternal And Child Health