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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Engineering, expression in transgenic plants and characterisation of E559, a rabies virus-neutralising monoclonal antibody
Journal of Infectious Diseases, Volume 210, No. 2, Year 2014
Notification
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Description
Rabies post-exposure prophylaxis (PEP) currently comprises administration of rabies vaccine together with rabies immunoglobulin (RIG) of either equine or human origin. In the developing world, RIG preparations are expensive, often in short supply, and of variable efficacy. Therefore, we are seeking to develop a monoclonal antibody cocktail to replace RIG. Here, we describe the cloning, engineering and production in plants of a candidate monoclonal antibody (E559) for inclusion in such a cocktail. The murine constant domains of E559 were replaced with human IgG1κ constant domains and the resulting chimeric mouse-human genes were cloned into plant expression vectors for stable nuclear transformation of Nicotiana tabacum. The plant-expressed, chimeric antibody was purified and biochemically characterized, was demonstrated to neutralize rabies virus in a fluorescent antibody virus neutralization assay, and conferred protection in a hamster challenge model. © 2014 The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4073784/bin/supp_210_2_200__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC4073784/bin/supp_jiu085_jiu085supp.docx
Authors & Co-Authors
Van Dolleweerd, Craig J.
United Kingdom, London
St George’s, University of London
Teh, Audrey Y.H.
United Kingdom, London
St George’s, University of London
Banyard, Ashley C.
United Kingdom, Addlestone
Animal and Plant Health Agency
Both, Leonard
United Kingdom, London
St George’s, University of London
Lotter, Therese
South Africa, Pretoria
The Council for Scientific and Industrial Research
Tsekoa, Tsepo Lebiletsa
South Africa, Pretoria
The Council for Scientific and Industrial Research
Phahladira, Baby M.N.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Shumba, Wonderful
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Chakauya, Ereck
South Africa, Pretoria
The Council for Scientific and Industrial Research
Sabeta, Claude T.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Gruber, Clemens
Austria, Vienna
Universitat Fur Bodenkultur Wien
Fooks, Anthony Richard
United Kingdom, Addlestone
Animal and Plant Health Agency
Chikwamba, Rachel K.
South Africa, Pretoria
The Council for Scientific and Industrial Research
Ma, Julian K.C.
United Kingdom, London
St George’s, University of London
Statistics
Citations: 48
Authors: 14
Affiliations: 5
Identifiers
Doi:
10.1093/infdis/jiu085
ISSN:
00221899
e-ISSN:
15376613
Research Areas
Cancer