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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Mutations within folate metabolising genes of Plasmodium falciparum in Cameroon
African Journal of Biotechnology, Volume 8, No. 19, Year 2009
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Description
Sulfadoxine-Pyrimethamine (S-P) still used in some parts of the country was suggested as the second line drug to amodiaquine following widespread failure of chloroquine in Cameroon in 2002. We investigated the efficacy of S-P and determined the baseline mutations on marker genes for folate metabolism (dhfr and dhps) in the forest and Guinea-Savanna ecozones of Cameroon, as a way of tracking resistance in patients aged between 0.5 and 10 years in Limbe (n=138), Nkambe (n=103), Fontem (n=100) and Dschang (n=93). Filter paper blood sample were collected prior to treatment and on clinical failure days to determine the prevalence of molecular markers of resistance and to assess the mutation rates on the folate metabolising genes by restriction fragment length polymorphism assays or dot-blot assays with 32-P labeled mutation-specific probes. Sequencing using the dideoxy-chain termination method by PCR was conducted to confirm doubtful cases. Late parasitological failure (LPF) was higher in Limbe (30.6%) compared to Nkambe 10.3% (p=0.001). The prevalence of the 437-Gly mutation though lower in Nkambe, 57.6%, than in Limbe, 60% were statistically not different (p=0.2). All genotypes with the 108N mutation also carried the 51-Ile and 59-Arg mutations. All sensitive alleles (S108) also carried the amino acids, 51-Asn and 59-Cys. S-P is no longer efficacious in Limbe and Nkambe, Cameroon for treating uncomplicated malaria in children below 10 years. Instead 437G rather than the 108 N of Plasmodium falciparum may be determinant as the marker for tracking the spread of SP resistance in Cameroon. © 2009 Academic Journals.
Authors & Co-Authors
Mbacham, Wilfred Fon
Cameroon, Yaounde
Université de Yaoundé I
Evehe, Marie Solange Bebandue
Cameroon, Yaounde
Université de Yaoundé I
Netongo, Palmer Masumbe
Cameroon, Yaounde
Université de Yaoundé I
Ali, Innocent Mbulli
Cameroon, Yaounde
Université de Yaoundé I
Nfor, Emmanuel
Cameroon, Yaounde
Université de Yaoundé I
Akaragwe, Ateh Isabel
Cameroon, Buea
University of Buea
Mimche, Patrice Nsangou
Cameroon, Yaounde
Université de Yaoundé I
Nji, Akindeh Mbuh
Cameroon, Yaounde
Université de Yaoundé I
Djoko, Cyrille Finyom
Cameroon, Yaounde
Université de Yaoundé I
Tawe, Bantar
Cameroon, Yaounde
Université de Yaoundé I
Gawa, Bidla
Cameroon, Buea
University of Buea
Asongna, Theresia
Cameroon, Yaounde
Université de Yaoundé I
Toh, Gang Boniface
Cameroon, Yaounde
Université de Yaoundé I
Atogho-Tieudeu, Barbara
Cameroon, Yaounde
Université de Yaoundé I
Nge, Nabi
Cameroon, Yaounde
Université de Yaoundé I
Ebeng, Raymond
Cameroon, Limbe
Bota District Hospital
Mokube, John Ahmadou
Cameroon, Limbe
Bota District Hospital
Kuaban, Christopher
Cameroon, Yaounde
Yaounde Jamot Hospital
Bickii, Jean
Cameroon, Yaounde
Institute of Medical Research and Medicinal Plant Studies Yaounde
Penlap, Véronique B.
Cameroon, Yaounde
Université de Yaoundé I
Titanji, Vincent Pryde Kehdinga
Cameroon, Buea
University of Buea
Njikam, N.
Cameroon, Yaounde
Université de Yaoundé I
Statistics
Citations: 22
Authors: 22
Affiliations: 5
Identifiers
e-ISSN:
16845315
Research Areas
Cancer
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study
Study Locations
Cameroon
Guinea