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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Binding of human serum amyloid P component (hSAP) to human neutrophils
European Journal of Biochemistry, Volume 223, No. 3, Year 1994
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Description
Human serum amyloid P component (hSAP) and human C‐reactive protein (hCRP) are normal serum constituents related to the pentraxin family of plasma proteins. hSAP has morphological and immunochemical identity and extensive sequence similarity to the amyloid P (AP) component found in normal tissues and particularly in amyloid deposits. hCRP and its proteolytic products have been previously shown to bind and to interact with various types of human leukocytes. Binding‐displacement experiments with 125I‐labeled hSAP and hCRP show that both proteins have specific high‐affinity binding sites on normal human polymorphonuclear leukocytes (PMN) and each can compete efficiently with the binding of the other. Scatchard analysis of hSAP‐displacement curves reveals a heterogeneous population of hSAP‐binding sites existing on the PMN cells, among them about 300000 low‐affinity binding sites with Kd≤ 5X10−6 M and about 30000 high‐affinity binding sites with Kd≤ 5X10−8 M. hAP was found to be degraded by enzymes from human neutrophils to yield a mixture of low‐molecular‐mass peptides, similarly to the case of CRP reported previously. The binding of hSAP can be efficiently inhibited by this peptide mixture. The results suggest that both hCRP and hSAP, together with related peptides, may participate in vivo in an unknown mechanism of regulation of human neutrophils. Copyright © 1994, Wiley Blackwell. All rights reserved
Authors & Co-Authors
LANDSMANN, Pavel
Israel, Rehovot
Weizmann Institute of Science Israel
ROSEN, Oren
Israel, Rehovot
Weizmann Institute of Science Israel
PONTET, Michel
France, Paris
Laboratoire de Chimie Biologique
PRAS, Mordechai
Israel, Tel Hashomer Tel Aviv
Heller Institute of Medical Research
LEVARTOWSKY, David
Israel, Tel Hashomer Tel Aviv
Heller Institute of Medical Research
Shephard, Enid G.
South Africa, Cape Town
Faculty of Health Sciences
Fridkin, Mati
Israel, Rehovot
Weizmann Institute of Science Israel
Statistics
Citations: 30
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1111/j.1432-1033.1994.tb19056.x
ISSN:
00142956
e-ISSN:
14321033
Study Design
Cross Sectional Study