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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of resistance to ivermectin in haemonchus contortus
International Journal for Parasitology, Volume 21, No. 7, Year 1991
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Description
Gill J. H., Redwin J. M., Wyk J. A. Van and Lacey E. 1991. Detection of resistance to ivermectin in Haemonchus contortus. International Journal for Parasitology 21: 771-776. Infective, third-stage (L3) larvae of Haemonchus contortus isolates resistant to ivermectin (IVM) show a decreased sensitivity to IVM-induced paralysis in vitro. The inhibition of larval motility by IVM can be detected in L3 larvae incubated in the dark on an agar matrix containing IVM, by the failure of affected larvae to move when stimulated by exposure to light. Optimally, avermectin (AVM) potency is quantified after three cycles, each involving storage in the dark for 24 h followed by a brief exposure to light. For IVM-susceptible isolates, a 50% inhibition of motility (LP50) was achieved with IVM concentrations between 0.30 and 0.49 μM, while LP50 values in IVM-resistant isolates ranged from 0.8 to 2.6 μM depending on the in vivo resistance status of the isolate. A limited study of structure-activity relationships within the AVM class indicated that in vitro inhibition of L3 motility was consistent with the known in vivo efficacy of each analogue. Resistance factors for IVM-resistant isolates were dependent on AVM structure with the more polar AVM B2 analogue being a particularly sensitive probe of IVM-resistance status. © 1991.
Authors & Co-Authors
Gill, Jennifer H.
Australia, Geelong
Csiro Livestock Industries
Redwin, Judith M.
Australia, Geelong
Csiro Livestock Industries
Van Wyk, J. A.
South Africa, Onderstepoort
Onderstepoort Veterinary Institute
Lacey, Ernest
Australia, Geelong
Csiro Livestock Industries
Statistics
Citations: 97
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/0020-7519(91)90144-V
ISSN:
00207519