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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Guanine-Plus-cytosine Content, hybridization percentages, and ecori restriction enzyme profiles of spiroplasmal dna
Reviews of Infectious Diseases, Volume 4, Year 1982
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Description
The guanine-plus-cytosine (G + C) content of spiroplasmal DNA was calculated from the melting temperature determined spectrophotometrically and the buoyant density determined by equilibrium density gradient centrifugation in CsCl. Only two ranges of G + C values were found: 25–27 mol% and 29–31 mol%. The DNA of the following spiroplasmas has 25–27 mol% G + C: Spiroplasma citri (serogroup 1-1); the spiroplasmas pathogenic to the honeybee (KC3, Be3, and B63; serogroup 1–2); the corn stunt strain (E275; serogroup 1-3); the tick strain 277F (serogroup l–4); the drosophila strain (serogroup II); and one group of flower spiroplasmas (serogroup III). The DNA of a second group of flower spiroplasmas (serogroup IV) and the SMCA strain (serogroup V) has a G + C content of 29–31 mol%. The classification of flower spiroplasmas into two groups on the basis of G + C content agrees well with the groupings based on serologic and protein analysis. Spiroplasmas isolated from honeybees in Morocco (B13) or froghoppers in Corsica (L89) have 29–31 mol% G + C, a value that corroborates the relatedness of these strains and the flower spiroplasmas of serogroup IV found by serologic analysis. Reannealing experiments between in vivo-labeled DNA of S. citri and unlabeled DNA of other spiroplasmas gave the following percentages of hybridization: 64% with honeybee spiroplasma DNA, 49% with corn stunt spiroplasma DNA, and 19% with tick spiroplasma 277F DNA; no significant hybridization was observed with DNA of any other spiroplasma. The taxonomic position of the tick spiroplasma 277F within serogroup I was confirmed by hybridization experiments involving [3H]DNA of this strain. The value of polyacrylamide gel analysis of DNA fragments produced by the action of EcoRI restriction enzyme on DNAs from various spiroplasmas is also discussed. © 1982 by The University of Chicago.
Authors & Co-Authors
Bové, Joseph Marie
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Saillard, C.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Junca, P.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
DeGorce-Dumas, J. R.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Ricard, Bérénice
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Nhami, A.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Whitcomb, R. F.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Williamson, D.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Tully, J. G.
France, Villenave-d'ornon
Centre Inrae Nouvelle-aquitaine Bordeaux
Morocco, Agdal Rabat
Société de Développement Agricole
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
United States, Stony Brook
Stony Brook University
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Statistics
Citations: 33
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1093/clinids/4.Supplement_1.S129
ISSN:
01620886
Research Areas
Genetics And Genomics
Study Locations
Morocco