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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Improvement of a pesticide immunosensor performance using site-directed antibody immobilisation and carbon nanotubes
International Journal of Nanotechnology, Volume 10, No. 5-7, Year 2013
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Description
The potential toxicity of pesticide residues in drinking water has meant a rigid regulation for the appearance of these pollutants. Thus, in this work, we developed a new immunosensor for atrazine detection. We focused on the optimisation of the antibody immobilisation method on sensor surface for the enhancement of the biosensor sensitivity. First, with site-directed immobilisation of rabbit anti-atrazine antibodies using goat anti-rabbit immunoglobulin, a detection limit of 0.5 ng/mL was obtained. This value is 20 times lower than the detection limit obtained with non-oriented antibodies. The second way to improve immunosensor sensitivity consisted of the addition of carbon nanotubes (CNT). As result of using these CNT, detection limit has been improved again from 0.5 ng/mL to 100 pg/mL. Copyright © 2013 Inderscience Enterprises Ltd.
Authors & Co-Authors
Marrakchi, Mouna
Tunisia, Tunis
University of Carthage
Helali, Saloua
Tunisia, Hamam Lif
Centre de Recherches et Des Technologies de L'energie
Camino, Juan Soto
Spain, Valencia
Universitat Politècnica de València
González-Martínez, Miguel Angel
Spain, Valencia
Universitat Politècnica de València
Abdelghani, Adnane
Tunisia, Tunis
University of Carthage
Hamdi, Moktar
Tunisia, Tunis
University of Carthage
Statistics
Citations: 8
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1504/IJNT.2013.053519
ISSN:
14757435
Research Areas
Environmental