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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Role of auxin during intercellular infection of Discaria trinervis by Frankia
Frontiers in Plant Science, Volume 5, No. AUG, Article 399, Year 2014
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Description
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses. © 2014 Imanishi, Perrine-Walker, Ndour, Vayssières, Conejero, Lucas, Champion, Laplaze, Wall and Svistoonoff.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4139986/bin/Presentation1.PDF
Authors & Co-Authors
Imanishi, Leandro
Argentina, Bernal
Universidad Nacional de Quilmes
France, Montpellier
Ird Centre de Montpellier
Perrine-Walker, Francine M.
France, Montpellier
Ird Centre de Montpellier
Ndour, Adama
Senegal, Dakar
Centre Ird de Bel Air
Vayssières, Alice
France, Montpellier
Ird Centre de Montpellier
Conéjéro, Geneviev̀e
France, Paris
Cirad
Lucas, Mikaël
France, Montpellier
Ird Centre de Montpellier
Champion, Antony
France, Montpellier
Ird Centre de Montpellier
Senegal, Dakar
Centre Ird de Bel Air
Laplaze, Laurent
France, Montpellier
Ird Centre de Montpellier
Senegal, Dakar
Centre Ird de Bel Air
Wall, Luis Gabriel
Argentina, Bernal
Universidad Nacional de Quilmes
Svistoonoff, Sergio
France, Montpellier
Ird Centre de Montpellier
Senegal, Dakar
Centre Ird de Bel Air
Statistics
Citations: 19
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.3389/fpls.2014.00399
e-ISSN:
1664462X
Research Areas
Genetics And Genomics