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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
In vitro anti-herpes simplex virus activity of crude extract of the roots of nauclea latifolia smith (rubiaceae)
BMC Complementary and Alternative Medicine, Volume 13, Article 266, Year 2013
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Description
Background: Nauclea latifolia Smith, a shrub belonging to the family Rubiaceae is a very popular medicinal plant in Cameroon and neighboring countries where it is used to treat jaundice, yellow fever, rheumatism, abdominal pains, hepatitis, diarrhea, dysentery, hypertension, as well as diabetes. The ethno-medicinal use against yellow fever, jaundice and diarrhea prompted us to investigate on the antiviral activity of the root bark of N. latifolia. In this study, HSV-2 was chosen as a viral model because of its strong impact on HIV transmission and acquisition. Methods: The crude extract under study was prepared by maceration of air-dried and powdered roots barks of N. latifolia in CH2Cl2/MeOH (50:50) mixture for 48 hours, then it was subjected to filtration and evaporation under vacuum. A phytochemical analysis of the crude extract was performed by High Performance Liquid Chromatography coupled with a photodiode array and mass spectrometry (HPLC-PDA-ESI-qMS). The anti-HSV-2 activity was assayed in vitro by plaque reduction and virus yield assays and the major mechanism of action was investigated by virucidal and time of addition assays. Data values were compared using the Extra sum of squares F test of program GraphPad PRISM 4.Results: The main components detected in the extract belong to the class of indole alkaloids characteristic of Nauclea genus. Strictosamide, vincosamide and pumiloside were tentatively identified together with quinovic acid glycoside. N. latifolia crude extract inhibited both acyclovir sensitive and acyclovir resistant HSV-2 strains, with IC50 values of 5.38 μg/ml for the former and 7.17 μg/ml for the latter. The extract was found to be most active when added post-infection, with IC50 of 3.63 μg/ml. Conclusion: The results of this work partly justify the empirical use of N. latifolia in traditional medicine for the treatment of viral diseases. This extract could be a promising rough material for the development of a new and more effective modern anti-HSV-2 medication also active against acyclovir-resistant HSV-2 strains. © 2013 Donalisio et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Donalisio, Manuela
Italy, Turin
Università Degli Studi Di Torino
Nana, H. Magnifouet
Italy, Turin
Università Degli Studi Di Torino
Cameroon, Dschang
University of Dschang
Ngono Ngane, Rosalie Annie
Cameroon, Douala
University of Douala
Gatsing, Donatien
Cameroon, Dschang
University of Dschang
Tiabou Tchinda, Alembert
Cameroon, Yaounde
Institute of Medical Research and Medicinal Plants Studies
Rovito, Roberta
Italy, Turin
Università Degli Studi Di Torino
Cagno, Valeria
Italy, Turin
Università Degli Studi Di Torino
Cagliero, Cecilia
Italy, Turin
Università Degli Studi Di Torino
Boyom, Fabrice Fekam
Cameroon, Yaounde
Université de Yaoundé I
Rubiolo, P.
Italy, Turin
Università Degli Studi Di Torino
Bicchi, Carlo P.
Italy, Turin
Università Degli Studi Di Torino
Lembo, David
Italy, Turin
Università Degli Studi Di Torino
Statistics
Citations: 51
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1186/1472-6882-13-266
e-ISSN:
14726882
Research Areas
Environmental
Health System And Policy
Infectious Diseases
Noncommunicable Diseases
Study Locations
Cameroon