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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Revealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes
Journal of Clinical Microbiology, Volume 49, No. 2, Year 2011
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Description
With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-HVR sequences. However, these strains showed considerable diversity within a larger sequencing window based on concatenated MV phosphoprotein and hemagglutinin genes (P/H pseudogenes). In Belarus, Germany, Russia, and the Democratic Republic of Congo, the P/H pseudogenes provided insights into chains of transmission, whereas identical NP-HVR provided none. In Russia, for instance, the P/H pseudogene identified temporal clusters rather than geographical clusters, demonstrating the circulation and importation of independent variants rather than large local outbreaks lasting for several years, as suggested by NP-HVR. Thus, by extending the sequencing window for molecular epidemiology, a more refined picture of MV circulation was obtained with more clearly defined links between outbreaks and transmission chains. Our results also suggested that in contrast to the P gene, the H gene acquired fixed substitutions that continued to be found in subsequent outbreaks, possibly with consequences for its antigenicity. Thus, a longer sequencing window has true benefits both for the epidemiological surveillance of measles and for the better monitoring of viral evolution. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3043479/bin/supp_49_2_677__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3043479/bin/supp_49_2_677__Supplementary_Material_Table.zip
Authors & Co-Authors
Kessler, Julia R.
Luxembourg, Strassen
Luxembourg Institute of Health
Kremer, Jacques R.
Luxembourg, Strassen
Luxembourg Institute of Health
Shulga, Sergey V.
Russian Federation, Moscow
Who Regional Reference Laboratory for Measles and Rubella
Tikhonova, Nina T.
Russian Federation, Moscow
Who Regional Reference Laboratory for Measles and Rubella
Santibanez, Sabine
Germany, Berlin
Who Regional Reference Laboratory for Measles and Rubella
Mankertz, Annette
Germany, Berlin
Who Regional Reference Laboratory for Measles and Rubella
Semeǐko, Galina V.
Belarus, Minsk
Who National Measles and Rubella Laboratory
Samoǐlovich, Elena O.
Belarus, Minsk
Who National Measles and Rubella Laboratory
Tamfum, Jean Jacques Muyembe
Democratic Republic Congo, Gombe, Kinshasa
Institut National de Recherche Biomédicale
Pukuta-Simbu, Elisabeth
Democratic Republic Congo, Gombe, Kinshasa
Institut National de Recherche Biomédicale
Muller, Claude P.
Luxembourg, Strassen
Luxembourg Institute of Health
Statistics
Citations: 18
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1128/JCM.01703-10
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Congo