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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Recombinant production and properties of binding of the full set of mouse secreted phospholipases A
2
to the mouse M-type receptor
Biochemistry, Volume 46, No. 6, Year 2007
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Description
To date, 12 secreted phospholipases A2 (sPLA2s) have been identified in the mouse species and divided into three structural collections (I/II/V/X, III, and XII). On the basis of their different molecular properties and tissue distributions, each sPLA2 is likely to exert distinct functions by acting as an enzyme or ligand for specific soluble proteins or receptors, among which the M-type receptor is the best-characterized target. Here, we present the properties of binding of the full set of mouse sPLA2s to the mouse M-type receptor. All enzymes have been produced in Escherichia coli or insect cells, and their properties of binding to the cloned and native M-type receptor have been determined. sPLA2s IB, IIA, IIE, IIF, and X are high-affinity ligands (K0.5 = 0.3-3 nM); sPLA2s IIC and V are low-affinity ligands (K0.5 = 30-75 nM), and sPLA2s IID, III, XIIA, and XIIB bind only very weakly or do not bind to the M-type receptor (K0.5 > 100 nM). Three exogenous parvoviral group XIII PLA2s and two fungal group XIV sPLA 2s do not bind to the receptor. Together, these results indicate that the mouse M-type receptor is selective for only a subset of mouse sPLA 2s from the group I/II/V/X structural collection. Binding of mouse sPLA2s to a recombinant soluble mouse M-type receptor leads in all cases to inhibition of enzymatic activity, and the extent of deglycosylation of the receptor decreases yet does not abolish sPLA2 binding. The physiological meaning of binding of sPLA2 to the M-type receptor is discussed on the basis of our current knowledge of sPLA2 functions. © 2007 American Chemical Society.
Authors & Co-Authors
Rouault, Morgane
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Le Calvez, Catherine
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Boilard, Eric
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Surrel, Fanny
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Singer, Alan G.
United States, Seattle
University of Washington
Ghomashchi, Farideh
United States, Seattle
University of Washington
Bezzine, Sofiane
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Scarzello, Sabine
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Bollinger, James G.
United States, Seattle
University of Washington
Gelb, Michael H.
United States, Seattle
University of Washington
Lambeau, Gérard
France, Valbonne
Institut de Pharmacologie Moléculaire et Cellulaire
Statistics
Citations: 11
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1021/bi062119b
Study Design
Phenomenological Study