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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Chitosan-hyaluronic acid nanoparticles for gene silencing: The role of hyaluronic acid on the nanoparticles' formation and activity
Colloids and Surfaces B: Biointerfaces, Volume 103, Year 2013
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Description
Hyaluronic acid (HA) has been described as a biocompatibility enhancer for gene delivery systems; however, the mechanistic implications of its inclusion on the formation and activity of such systems and subsequent gene release are poorly understood. To better understand these issues, we describe herein the preparation and characterization of chitosan and chitosan-hyaluronic acid nanoparticles (CS and CS:HA NPs) for gene silencing. Different formulations were prepared by ionotropic gelation and evaluated for their physicochemical properties and biological activities in A549-Luc cells. Inclusion of HA to CS NPs resulted in a comparable silencing activity with Lipofectamine™ RNAiMAX (≈85% of luciferase knockdown) and significantly improved cell viability compared with CS NPs. As depicted by isothermal titration calorimetry, HA competed with siRNA for CS binding, lowering CS-siRNA binding strength by 25%. This suggests that besides improving cell biocompatibility of CS NPs, HA might also promote their gene release by loosening the CS-siRNA binding. © 2012 Elsevier B.V.
Authors & Co-Authors
Al-Qadi, Sonia
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Alatorre-Meda, Manuel
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Zaghloul, Eman M.
Sweden, Stockholm
Karolinska Institutet
Egypt, Alexandria
Faculty of Pharmacy
Taboada, Pablo
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Remunán-López, Carmen
Spain, Santiago de Compostela
Universidad de Santiago de Compostela
Statistics
Citations: 82
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.colsurfb.2012.11.009
ISSN:
09277765
e-ISSN:
18734367
Research Areas
Genetics And Genomics