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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparison of mecA polymerase chain reaction with phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus
Current Microbiology, Volume 55, No. 6, Year 2007
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Description
In the present study, several conventional methods to detect methicillin-resistant Staphylococcus aureus (MRSA) were compared with polymerase chain reaction (PCR) detection of mecA gene-positive isolates. Cefoxitin E-test was also evaluated as a possible phenotypic method of MRSA detection. Oxacillin agar screen and PBP2′ latex agglutination methods were found to be more sensitive than oxacillin and cefoxitin disk-diffusion methods. Cefoxitin disk diffusion was found to be the most specific. A combination of oxacillin agar screening with cefoxitin disk diffusion, or oxacillin disk diffusion with PBP2′, improved sensitivity and specificity. Cefoxitin E-test with the current break points had low sensitivity and specificity (33.3% and 75%, respectively) for the detection of MRSA. However, changing the break points to ≤4 μg/ml and to ≥6 μg/ml for sensitive and resistant, respectively, greatly improved both. Changing the 30-μg cefoxitin disk-diffusion break points to ≤21 mm for resistant slightly improved sensitivity but had no effect on specificity. It was therefore concluded that the use of more than one screening method is necessary to detect all MRSA isolates in clinical settings. © 2007 Springer Science+Business Media, LLC.
Authors & Co-Authors
Baddour, Manal M.
Egypt, Alexandria
Faculty of Medicine
Saudi Arabia, Riyadh
King Saud University
Abuelkheir, Manal M.
Saudi Arabia, Riyadh
King Saud University
Fatani, Amal Jamil Y.
Saudi Arabia, Riyadh
College of Pharmacy
Statistics
Citations: 78
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1007/s00284-007-9015-6
ISSN:
03438651
e-ISSN:
14320991
Research Areas
Genetics And Genomics